Aspergillus sporulation and mycotoxin production both require inactivation of the FadA G alpha protein-dependent signaling pathway
| Autor: | Thomas H. Adams, Nancy P. Keller, Jae-Hyuk Yu, Julie K. Hicks |
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| Rok vydání: | 1997 |
| Předmět: |
GTPase-activating protein
Transcription Genetic Sterigmatocystin Genes Fungal Asexual sporulation General Biochemistry Genetics and Molecular Biology Aspergillus nidulans Fungal Proteins Aflatoxins GTP-Binding Proteins Gene Expression Regulation Fungal RNA Messenger Molecular Biology Gene Transcription factor Regulation of gene expression Genetics Fungal protein General Immunology and Microbiology biology General Neuroscience fungi GTPase-Activating Proteins Fungal genetics Proteins Spores Fungal biology.organism_classification Aspergillus Mutation Chromatography Thin Layer Signal Transduction Research Article |
| Zdroj: | The EMBO journal. 16(16) |
| ISSN: | 0261-4189 |
| Popis: | The filamentous fungus Aspergillus nidulans contains a cluster of 25 genes that encode enzymes required to synthesize a toxic and carcinogenic secondary metabolite called sterigmatocystin (ST), a precursor of the better known fungal toxin aflatoxin (AF). One ST Cluster (stc) gene, aflR, functions as a pathway-specific transcriptional regulator for activation of other genes in the ST pathway. However, the mechanisms controlling activation of aflR and synthesis of ST and AF are not understood. Here we show that one important level for control of stc gene expression requires genes that were first identified as early acting regulators of asexual sporulation. Specifically, we found that loss-of-function mutations in flbA, which encodes a RGS domain protein, or dominant activating mutations in fadA, which encodes the alpha subunit of a heterotrimeric G protein, block both ST production and asexual sporulation. Moreover, overexpression of flbA or dominant interfering fadA mutations cause precocious stc gene expression and ST accumulation, as well as unscheduled sporulation. The requirement for flbA in sporulation and ST production could be suppressed by loss-of-function fadA mutations. The ability of flbA to activate stc gene expression was dependent upon another early acting developmental regulator, fluG, and AflR, the stc gene-specific transcription factor. These results are consistent with a model in which both asexual sporulation and ST production require inactivation of proliferative growth through inhibition of FadA-dependent signaling. This regulatory mechanism is conserved in AF-producing fungi and could therefore provide a means of controlling AF contamination. |
| Databáze: | OpenAIRE |
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