| Autor: |
Peter L. Privalov, A.A. Bayev, T.V. Venkstern, Vladimir V. Filimonov |
| Rok vydání: |
1975 |
| Předmět: |
|
| Zdroj: |
Journal of Molecular Biology. 97:279-288 |
| ISSN: |
0022-2836 |
| DOI: |
10.1016/s0022-2836(75)80041-6 |
| Popis: |
Melting of purified tRNA1Val was investigated by scanning microcalorimetry under various salt conditions. It is shown that the total enthalpy of melting is 370 kcal mol−1 and depends only slightly on salt conditions and temperature. Such a high enthalpy is in good agreement with recent results of structural analysis, according to which more than 82% of bases are bonded in native tRNA. The thermodynamic analysis of the complicated melting curves shows that there are six essentially independent co-operative regions in tRNA structure; some of these regions merge in the presence of magnesium ions. A structural description of these regions is given. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
