Distribution and accumulation of 10 nm silver nanoparticles in maternal tissues and visceral yolk sac of pregnant mice, and a potential effect on embryo growth
| Autor: | Qin Zhang, Saber M. Hussain, Georgia K. Hinkley, Brendan J. Casey, Bridget Wildt, Thomas H. Umbreit, Martha W. Betz, Peter L. Goering, Anurag R. Mishra, Carlye A. Austin, Stephen M. Roberts, Sabine Francke-Carroll, Ken M. Brown |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Silver Materials science Biomedical Engineering Embryonic Development Metal Nanoparticles Gestational Age Spleen 010501 environmental sciences Kidney Toxicology 01 natural sciences Silver nanoparticle Andrology Mice 03 medical and health sciences chemistry.chemical_compound Pregnancy medicine Animals Tissue Distribution Yolk sac Yolk Sac 0105 earth and related environmental sciences Fetus Embryo Viscera Silver nitrate 030104 developmental biology medicine.anatomical_structure chemistry Maternal Exposure embryonic structures Silver Nitrate Gestation Female Histopathology |
| Zdroj: | Nanotoxicology. 10:654-661 |
| ISSN: | 1743-5404 1743-5390 |
| DOI: | 10.3109/17435390.2015.1107143 |
| Popis: | We examined the distribution of silver in pregnant mice and embryos/fetuses following intravenous injections of 10 nm silver nanoparticles (AgNPs) or soluble silver nitrate (AgNO3) at dose levels of 0 (citrate buffer control) or 66 µg Ag/mouse to pregnant mice on gestation days (GDs) 7, 8 and 9. Selected maternal tissues and all embryos/fetuses from control, AgNP- and AgNO3-treated groups on GD10 and control and AgNP-treated groups on GD16 were processed for the measurement of silver concentrations, intracellular AgNP localization, histopathology and gross examination of tissue morphology. Inductively-coupled plasma mass spectrometry revealed silver in all examined tissues following either AgNP or AgNO3 treatment, with highest concentrations of silver in maternal liver, spleen and visceral yolk sac (VYS), and lowest concentrations in embryos/fetuses. For VYS, mean silver concentration following AgNO3 treatment (4.87 ng Ag/mg tissue) was approximately two-fold that following AgNP treatment (2.31 ng Ag/mg tissue); for all other tissues examined, mean silver concentrations following either AgNP or AgNO3 treatment were not significantly different from each other (e.g. 2.57 or 2.84 ng Ag/mg tissue in maternal liver and 1.61 or 2.50 ng Ag/mg tissue in maternal spleen following AgNP or AgNO3 treatment, respectively). Hyperspectral imaging revealed AgNP aggregates in maternal liver, kidney, spleen and VYS from AgNP-treated mice, but not AgNO3-treated mice. Additionally, one or more embryos collected on GD10 from eight of ten AgNP-treated mice appeared small for their age (i.e. Theiler stage 13 [GD8.5] or younger). In the control group (N = 11), this effect was seen in embryos from only one mouse. In conclusion, intravenous injection of 10 nm AgNPs to pregnant mice resulted in notable silver accumulation in maternal liver, spleen and VYS, and may have affected embryonic growth. Silver accumulation in embryos/fetuses was negligible. |
| Databáze: | OpenAIRE |
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