Amperometric aptasensor with sandwich-type architecture for troponin I based on carboxyethylsilanetriol-modified graphene oxide coated electrodes
| Autor: | Reynaldo Villalonga, Beatriz Mayol, Itziar Estabiel, Ana M. Pérez-Calabuig, Concepción Parrado, Anabel Villalonga |
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| Rok vydání: | 2020 |
| Předmět: |
Materials science
Aptamer Biomedical Engineering Biophysics Nanotechnology 02 engineering and technology Biosensing Techniques 01 natural sciences law.invention Nanomaterials law Limit of Detection Electrochemistry Humans Electrodes Detection limit Graphene 010401 analytical chemistry Troponin I Reproducibility of Results General Medicine Electrochemical Techniques Aptamers Nucleotide 021001 nanoscience & nanotechnology Amperometry 0104 chemical sciences Electrode Graphite Gold 0210 nano-technology Biosensor Biotechnology Conjugate |
| Zdroj: | Biosensorsbioelectronics. 183 |
| ISSN: | 1873-4235 |
| Popis: | A novel amperometric aptasensor for the specific detection of cardiac troponin I (cTnI) was constructed by using screen-printed carbon electrodes coated with a carboxyethylsilanetriol-modified graphene oxide derivative as transduction element. This novel carboxylic acid-enriched nanomaterial allows easy and high load immobilization of the capture aptamer molecules on the electrode surface. The biosensing interface was assembled by covalent attachment of an amino-functionalized DNA aptamer on the carboxylic acid-enriched electrode surface. The sensing approach relies on the specific recognition of cTnI by the aptamer and further assembly of a sandwich-type architecture with a novel aptamer-peroxidase conjugate as signaling element. The aptasensor was employed to detect the cardiac biomarker in the broad range from 1.0 pg/mL to 1.0 μg/mL with a detection limit of 0.6 pg/mL. This electroanalytical device also showed high specificity, reproducibility and stability, and was useful to quantify cTnI in reconstituted human serum samples. |
| Databáze: | OpenAIRE |
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