Screening of IR50 x Rathu Heenati F7 RILs and identification of SSR markers linked to brown planthopper (Nilaparvata lugens Stål) resistance in rice (Oryza sativa L.)
| Autor: | Mohankumar Subbarayalu, Senthil Natesan, Maheshwaran Marappan, Nagarajan Pothi, Sanju Kumari, Jennifer M. Sheba, Suresh Seetharaman, Raveendran Muthurajan, Shanmugasunderam Ponnuswamy |
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| Rok vydání: | 2010 |
| Předmět: |
Genetic Markers
Genotype Genetic Linkage Plant genetics Quantitative Trait Loci Bioengineering Minisatellite Repeats Biology Quantitative trait locus Genes Plant Applied Microbiology and Biotechnology Biochemistry Hemiptera Animals Inbreeding Cultivar Genetic Testing Molecular Biology Crosses Genetic Plant Diseases Genetics Recombination Genetic Oryza sativa Polymorphism Genetic food and beverages Oryza Marker-assisted selection biology.organism_classification Immunity Innate Horticulture Genetic marker Microsatellite Brown planthopper Biotechnology |
| Zdroj: | Molecular biotechnology. 46(1) |
| ISSN: | 1559-0305 |
| Popis: | Brown planthopper (Nilaparvata lugens Stål) is one of the major insect pests of rice. A Sri Lankan indica rice cultivar Rathu Heenati was found to be resistant to all biotypes of the brown planthopper. In the present study, a total of 268 F(7) RILs of IR50 and Rathu Heenati were phenotyped for their level of resistance against BPH by the standard seedbox screening test (SSST) in the greenhouse. A total of 53 SSR primers mapped on the chromosome 3 were used to screen the polymorphism between the parents IR50 and Rathu Heenati, out of which eleven were found to be polymorphic between IR50 and Rathu Heenati. The eleven primers that have shown polymorphism between the IR50 and Rathu Heenati parents were genotyped in a set of five resistant RILs and five susceptible RILs along with the parents for co-segregation analysis. Among the eleven primers, two primers namely RM3180 (18.22 Mb) and RM2453 (20.19 Mb) showed complete co-segregation with resistance. The identification of SSR markers linked with BPH resistant could be used for the maker assisted selection (MAS) program in rice breeding and to map the resistant genes on rice chromosomes for further gene cloning. |
| Databáze: | OpenAIRE |
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