A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS
| Autor: | Laurina Manning, Sen-Lin Lai, Albert Cardona, Chris Q. Doe, Ashok Litwin-Kumar, Aref Arzan Zarin, Heather Q Pollington, James W Truman, Brandon Mark |
|---|---|
| Přispěvatelé: | Lai, Sen-Lin [0000-0002-7531-283X], Zarin, Aref Arzan [0000-0003-0484-3622], Litwin-Kumar, Ashok [0000-0003-2422-6576], Cardona, Albert [0000-0003-4941-6536], Truman, James W [0000-0002-9209-5435], Doe, Chris Q [0000-0001-5980-8029], Apollo - University of Cambridge Repository |
| Rok vydání: | 2021 |
| Předmět: |
Nervous system
Central Nervous System Notch QH301-705.5 Science Neurogenesis Central nervous system Sensory system neural circuit Biology hemilineage General Biochemistry Genetics and Molecular Biology Neuroblast Neural Stem Cells medicine Neuropil Animals Drosophila Proteins Biology (General) cell lineage General Immunology and Microbiology D. melanogaster General Neuroscience General Medicine medicine.anatomical_structure Drosophila melanogaster nervous system Connectome Medicine Developmental biology Neuroscience neuroblast temporal identity Research Article Developmental Biology |
| Zdroj: | eLife, Vol 10 (2021) |
| DOI: | 10.17863/cam.70301 |
| Popis: | Funder: HHMI; FundRef: http://dx.doi.org/10.13039/100000011 The mechanisms specifying neuronal diversity are well characterized, yet it remains unclear how or if these mechanisms regulate neural circuit assembly. To address this, we mapped the developmental origin of 160 interneurons from seven bilateral neural progenitors (neuroblasts) and identify them in a synapse-scale TEM reconstruction of the Drosophila larval central nervous system. We find that lineages concurrently build the sensory and motor neuropils by generating sensory and motor hemilineages in a Notch-dependent manner. Neurons in a hemilineage share common synaptic targeting within the neuropil, which is further refined based on neuronal temporal identity. Connectome analysis shows that hemilineage-temporal cohorts share common connectivity. Finally, we show that proximity alone cannot explain the observed connectivity structure, suggesting hemilineage/temporal identity confers an added layer of specificity. Thus, we demonstrate that the mechanisms specifying neuronal diversity also govern circuit formation and function, and that these principles are broadly applicable throughout the nervous system. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|