Biomolecular changes and subsequent time-dependent recovery in hippocampal tissue after experimental mild traumatic brain injury
| Autor: | Feride Severcan, Rick M. Dijkhuizen, Caroline L. van Heijningen, Mohamed H. M. Ali, Erwin L. A. Blezer, Fazle Rakib, Sebnem Garip Ustaoglu |
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| Přispěvatelé: | Garip Ustaoğlu, Şebnem, Severcan, Feride |
| Rok vydání: | 2021 |
| Předmět: |
Male
medicine.medical_specialty Traumatic Brain Injury Time Factors Traumatic brain injury Membrane Fluidity Science Hippocampus Hippocampal formation Brain injuries 01 natural sciences Protein Structure Secondary Article 03 medical and health sciences 0302 clinical medicine Injury Severity Score Internal medicine Biophysical chemistry TBI Brain Injuries Traumatic Spectroscopy Fourier Transform Infrared Membrane fluidity Sprague dawley rats Medicine Animals Humans Mild form Multidisciplinary business.industry 010401 analytical chemistry medicine.disease Lipid Metabolism Magnetic Resonance Imaging Lipids 0104 chemical sciences Rats Disease Models Animal Band area Endocrinology Acyl chain business Positron Emission Tomography 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-13 (2021) |
| ISSN: | 2045-2322 |
| Popis: | Traumatic brain injury (TBI) is the main cause of disability and mortality in individuals under the age of 45 years. Elucidation of the molecular and structural alterations in brain tissue due to TBI is crucial to understand secondary and long-term effects after traumatic brain injury, and to develop and apply the correct therapies. In the current study, the molecular effects of TBI were investigated in rat brain at 24 h and 1 month after the injury to determine acute and chronic effects, respectively by Fourier transform infrared imaging. This study reports the time-dependent contextual and structural effects of TBI on hippocampal brain tissue. A mild form of TBI was induced in 11-week old male Sprague Dawley rats by weight drop. Band area and intensity ratios, band frequency and bandwidth values of specific spectral bands showed that TBI causes significant structural and contextual global changes including decrease in carbonyl content, unsaturated lipid content, lipid acyl chain length, membrane lipid order, total protein content, lipid/protein ratio, besides increase in membrane fluidity with an altered protein secondary structure and metabolic activity in hippocampus 24 h after injury. However, improvement and/or recovery effects in these parameters were observed at one month after TBI. |
| Databáze: | OpenAIRE |
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