Anti-Herpes Simplex Virus Activity of Alkaloids Isolated from Stephania cepharantha
| Autor: | Minoru Ono, As'ari Nawawi, Masao Hattori, Noriaki Kashiwaba, Chao-Mei Ma, Kimiyasu Shiraki, Norio Nakamura, Masahiko Kurokawa |
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| Rok vydání: | 1999 |
| Předmět: |
viruses
Drug Evaluation Preclinical Pharmaceutical Science medicine.disease_cause Antiviral Agents Thymidine Kinase Virus law.invention chemistry.chemical_compound Alkaloids law Chlorocebus aethiops medicine Animals Simplexvirus Stephania Benzylisoquinoline Vero Cells IC50 Pharmacology Molecular Structure biology Traditional medicine Alkaloid General Medicine biology.organism_classification Herpes simplex virus chemistry Thymidine kinase Phytotherapy Drugs Chinese Herbal |
| Zdroj: | Biological and Pharmaceutical Bulletin. 22:268-274 |
| ISSN: | 1347-5215 0918-6158 |
| DOI: | 10.1248/bpb.22.268 |
| Popis: | By screening water and MeOH extracts of 30 Chinese medicinal plants for their anti-herpes simplex virus (HSV)-1 activity, a MeOH extract of the root tubers of Stephania cepharantha HAYATA showed the most potent activity on the plaque reduction assay with an IC50 value of 18.0 microg/ml. Of 49 alkaloids isolated from the MeOH extract, 17 alkaloids were found to be active against HSV-1, including 13 bisbenzylisoquinoline, 1 protoberberine, 2 morphinane and 1 proaporphine alkaloids, while benzylisoquinoline and hasubanane alkaloids were inactive. Although N-methylcrotsparine was active against HSV-1, as well as HSV-1 thymidine kinase deficient (acyclovir resistant type, HSV-1 TK-) and HSV-2 (IC50 values of 8.3, 7.7 and 6.7 microg/ml, respectively), it was cytotoxic. FK-3000 was found to be the most active against HSV-1, HSV-1 TK- and HSV-2 (IC50 values of 7.8, 9.9 and 8.7 microg/ml) with in vitro therapeutic indices of 90, 71 and 81, respectively. FK-3000 was found to be a promising candidate as an anti-HSV agent against HSV-1, acyclovir (ACV) resistant-type HSV-1 and HSV-2. |
| Databáze: | OpenAIRE |
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