Sirolimus and propranolol inhibit endothelial proliferation while detergent sclerosants induce endothelial activation, microparticle release and apoptosis in vitro
| Autor: | Kelvin Cheung, Osvaldo Cooley-Andrade, Dulani Goonawardhana, Kurosh Parsi, Jessica Gerbelli, An-Ning Chew, David E. Connor |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Detergents
Apoptosis Propranolol 030204 cardiovascular system & hematology Pharmacology Sodium Tetradecyl Sulfate Endothelial activation Polidocanol Mice 03 medical and health sciences 0302 clinical medicine Human Umbilical Vein Endothelial Cells medicine Animals Humans Cell Proliferation Sirolimus business.industry General Medicine Microparticle release Sclerosing Solutions In vitro Endothelial stem cell 030220 oncology & carcinogenesis Cardiology and Cardiovascular Medicine business medicine.drug |
| Zdroj: | Phlebology: The Journal of Venous Disease. 35:566-575 |
| ISSN: | 1758-1125 0268-3555 |
| DOI: | 10.1177/0268355520913384 |
| Popis: | Objectives To investigate the effects of detergent sclerosants, sodium tetradecyl sulphate and polidocanol, on endothelial cell activation and microparticle release and the effects of detergent sclerosants, sirolimus and propranolol, on apoptosis in vitro. Methods Cultured human umbilical vein endothelial cells and murine haemangioendothelioma (EOMA) cell lines were incubated with different concentrations of sodium tetradecyl sulphate and polidocanol, as well as sirolimus and propranolol. Endothelial activation was assessed using flow cytometry for CD62e (E-Selectin), CD54 (ICAM-1), CD105 (endoglin), CD144 (VE-Cadherin), CD146 (MCAM) and the release of endothelial microparticles. Cell proliferation was assessed using [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] and carboxyfluorescein succinimidyl ester assays. Apoptosis was assessed using flow cytometry for lactadherin/propidium iodide staining and for Caspase-3 expression. Results Sublytic concentrations of sodium tetradecyl sulphate and polidocanol (0.075%–0.3%) increased the expression of the activation markers CD62e and CD54. The expression of CD105 decreased in sclerosant treated cultured human umbilical vein endothelial cells. Both sodium tetradecyl sulphate and polidocanol induced the release of endothelial microparticles. All agents inhibited cell proliferation. Sodium tetradecyl sulphate and polidocanol-induced apoptosis as evidenced by increased phosphatidylserine exposure and caspase-3 expression, whereas sirolimus and propranolol increased caspase-3 expression only. Conclusion Sublytic concentrations of detergent sclerosants induce endothelial activation and the release of endothelial microparticles. All agents were anti-proliferative in EOMA cell lines, with sodium tetradecyl sulphate and polidocanol inducing cellular apoptosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|