An Introduction to Performing Immunofluorescence Staining
| Autor: | Kyuseok Im, M Fernando Palma Diaz, Sergey Mareninov, William H. Yong |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Fluorophore
Immunocytochemistry Fluorescent Antibody Technique Immunofluorescence Antibodies Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Antigen Microscopy medicine Humans Antigens Fluorescent Antibody Technique Indirect 030304 developmental biology 0303 health sciences Staining and Labeling medicine.diagnostic_test Chemistry Immunohistochemistry Molecular biology Staining Antigen retrieval 030220 oncology & carcinogenesis |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493989331 |
| Popis: | Immunofluorescence (IF) is an important immunochemical technique that allows for detection and localization of a wide variety of antigens in different types of tissues of various cell preparations. IF allows for excellent sensitivity and amplification of signal in comparison to immunohistochemistry, employing various microscopy techniques. There are two methods available, depending on the scope of the experiment or the specific antibodies in use: direct (primary) or indirect (secondary). Here, we describe preparation of specimens preserved in different types of media and step-by-step methods for both direct and indirect immunofluorescence staining. |
| Databáze: | OpenAIRE |
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