JNK signaling pathway regulates the development of ovaries and synthesis of vitellogenin (Vg) in the swimming crab Portunus trituberculatus
| Autor: | Xing Li, Zhiming Ren, Chunlin Wang, Lei Tang, Ce Shi, Hongzhi Yao, Hongling Wei, Changkao Mu, Huan Wang |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway Brachyura MAP Kinase Signaling System Biochemistry Vitellogenins 03 medical and health sciences chemistry.chemical_compound Vitellogenin 0302 clinical medicine Sequence Analysis Protein Animals Cloning Molecular Protein kinase A Anisomycin Original Paper biology Activator (genetics) Kinase Ovary JNK Mitogen-Activated Protein Kinases Cell Biology Portunus trituberculatus biology.organism_classification Cell biology 030104 developmental biology chemistry Apoptosis 030220 oncology & carcinogenesis biology.protein Female Sequence Alignment |
| Zdroj: | Cell Stress Chaperones |
| ISSN: | 1466-1268 1355-8145 |
| DOI: | 10.1007/s12192-020-01085-1 |
| Popis: | The development of Portunus trituberculatus egg cells is directly related to the nutritional status of the fertilized egg, which affects the key production stages of offspring hatching. Vitellogenin plays a key role in the nutrient supply required for the development of the egg cells. The c-Jun N-terminal kinase (JNK) is an important member of the mitogen-activated protein kinase (MAPK) superfamily and plays an important role in cell proliferation, transformation, differentiation, and apoptosis. At present, there are no reports on the involvement of the JNK signaling pathway in the reproductive regulation of P. trituberculatus. In this study, rapid amplification of complementary DNA ends amplification technology was used to clone the full length of JNK complementary DNA, which has a length of 2094 bp, including an open reading frame (ORF) of 1266 bp encoding a 421-amino acid protein. The protein includes the S_TKC conserved domain with a TPY phosphorylation site, which is a typical feature of the JNK gene family. Observing tissue sections found the oocytes in the inhibitor group developed slowly, while the oocytes in the activated group showed accelerated development. Meanwhile, Portunus trituberculatus JNK and vitellogenin (Vg) genes exhibited the same trend in the hepatopancreas and ovaries, and the expression of the SP600125 group was downregulated (P |
| Databáze: | OpenAIRE |
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