Unravelling a direct role for polysaccharide β-strands in the higher order structure of physical hydrogels
| Autor: | Christian Johannessen, Aurelien Forget, Rina K. Dukor, Steffen Lüdeke, Anjan Roy, V. Prasad Shastri, Florian Mießmer, Carolina Carballo, Anja Rüther, Laurence A. Nafie |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
chemistry.chemical_classification
Stereochemistry General Medicine 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology Polysaccharide 01 natural sciences Oligomer Catalysis Spectral line 0104 chemical sciences chemistry.chemical_compound Chemistry chemistry Carboxylation Chemical engineering Phase (matter) Self-healing hydrogels Agarose Raman optical activity 0210 nano-technology |
| Zdroj: | Angewandte Chemie: international edition in English |
| ISSN: | 1433-7851 |
| Popis: | The mechanical properties of agarose-derived hydrogels depend on the scaffolding of the polysaccharide network. To identify and quantify such higher order structure, we applied Raman optical activity (ROA)a spectroscopic technique that is highly sensitive toward carbohydrateson native agarose and chemically modified agarose in the gel phase for the first time. By spectral global fitting, we isolated features that change as a function of backbone carboxylation (28, 40, 50, 60, 80, and 93 %) from other features that remain unchanged. We assigned these spectral features by comparison to ROA spectra calculated for different oligomer models. We found a 60:40 ratio of double- and single-stranded α-helix in the highly rigid hydrogel of native agarose, while the considerably softer hydrogels made from carboxylated agarose use a scaffold of unpaired β-strands. |
| Databáze: | OpenAIRE |
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