The effect of single-file reciprocating systems on Substance P and Calcitonin gene-related peptide expression in human periodontal ligament
| Autor: | R. Delgado, J. Caviedes-Bucheli, C. P. Carreño, Jaime Omar Moreno, J. Solano, D. J. Garcia, E. Diaz, Hugo Roberto Muñoz |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
medicine.medical_specialty Materials science Adolescent Periodontal Ligament Calcitonin Gene-Related Peptide Root canal Positive control Dentistry Substance P Calcitonin gene-related peptide Young Adult chemistry.chemical_compound stomatognathic system Internal medicine medicine Humans Periodontal fiber Bicuspid General Dentistry Neurotransmitter Agents Neurogenic inflammation business.industry Radioimmunoassay Equipment Design medicine.anatomical_structure Endocrinology chemistry Calcitonin business Root Canal Preparation |
| Zdroj: | International Endodontic Journal. 46:419-426 |
| ISSN: | 0143-2885 |
| DOI: | 10.1111/iej.12005 |
| Popis: | Aim To quantify the effect of two single-file reciprocating root canal preparation systems on Substance P (SP) and Calcitonin gene-related peptide (CGRP) expression in healthy human periodontal ligament (PDL). Methodology Forty PDL samples were obtained from healthy premolars where extraction was indicated for orthodontic reasons. Prior to extraction, 20 of these premolars were divided equally in two groups, and then, root canals were prepared using one of two different single-file systems: WaveOne and Reciproc. Ten premolars were prepared with hand files and served as a positive control group. The remaining 10 premolars where extracted without treatment and served as a negative control group. All PDL samples were processed, and SP and CGRP were measured by radioimmunoassay. Results Greater SP and CGRP expression were found in the hand instrumentation group (1.220 pmol SP and 0.084 pmol CGRP per mg of PDL), followed by the WaveOne group (0.908 pmol SP and 0.046 pmol CGRP per mg of PDL) and the Reciproc group (0.511 pmol SP and 0.022 pmol CGRP per mg of PDL). The lower SP and CGRP values were associated with the intact control group (0.453 pmol SP and 0.018 pmol CGRP per mg of PDL). The Kruskal–Wallis test revealed significant differences between groups (P |
| Databáze: | OpenAIRE |
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