A Novel Thermal Bubble Valve Integrated Nanofluidic Preconcentrator for Highly Sensitive Biomarker Detection
Autor: | Chih Zong Deng, Pei-Shan Chung, Horn-Jiunn Sheen, Yu Jui Fan |
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Rok vydání: | 2018 |
Předmět: |
Control valves
Materials science Bioengineering 02 engineering and technology 01 natural sciences Diffusion Electrokinetic phenomena Lab-On-A-Chip Devices medicine Humans Instrumentation Immunoassay Fluid Flow and Transfer Processes Detection limit Microchannel medicine.diagnostic_test business.industry Process Chemistry and Technology 010401 analytical chemistry Equipment Design Microfluidic Analytical Techniques 021001 nanoscience & nanotechnology Coronary heart disease 0104 chemical sciences Highly sensitive C-Reactive Protein Sound Thermal bubble Optoelectronics 0210 nano-technology business Antibodies Immobilized |
Zdroj: | ACS Sensors. 3:1409-1415 |
ISSN: | 2379-3694 |
Popis: | In this study, we developed a new immunosensor that can achieve an ultralow detection limit and high sensitivity. This new device has an electrokinetic trapping (EKT)-based nanofluidic preconcentrator, which was integrated with oscillating bubble valves, to trap concentrated antigen and immunobeads. During the immunoassay process, oscillating bubbles rapidly grew and acted as control valves and to block the microchannel. Thereafter, the trapped preconcentrated antigen plug and antibody-coated nanobeads were preserved in the region between these two valves. Finally, the antigen concentration was quantitatively analyzed by a real-time measurement of Brownian diffusion of the immunobeads. In this work, the test sample used was C-reactive protein (CRP) which is a risk indicator of coronary heart disease and atherosclerosis. |
Databáze: | OpenAIRE |
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