Whole transcriptome data analysis of mouse embryonic hematopoietic stem and progenitor cells that lack Geminin expression
| Autor: | Christos Papadimitriou, Stavros Taraviras, Nikolaos I. Kanellakis, Alexandra L. Patmanidi, Zoi Lygerou, Dimitris Karamitros |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Multidisciplinary biology Microarray Geminin lcsh:Computer applications to medicine. Medical informatics Embryonic stem cell Molecular biology Transcriptome 03 medical and health sciences Haematopoiesis 030104 developmental biology 0302 clinical medicine 030220 oncology & carcinogenesis embryonic structures biology.protein lcsh:R858-859.7 Stem cell Progenitor cell lcsh:Science (General) Gene lcsh:Q1-390 Data Article |
| Zdroj: | Data in Brief Data in Brief, Vol 7, Iss, Pp 889-893 (2016) |
| ISSN: | 2352-3409 |
| Popis: | We performed cDNA microarrays (Affymetrix Mouse Gene 1.0 ST Chip) to analyze the transcriptome of hematopoietic stem and progenitor cells (HSPCs) from E15.5dpc wild type and Geminin (Gmnn) knockout embryos. Lineage negative cells from embryonic livers were isolated using fluorescence activated cell sorting. RNA samples were used to examine the transcriptional programs regulated by Geminin during embryonic hematopoiesis. The data sets were analyzed using the GeneSpring v12.5 platform (Agilent). The list of differentially expressed genes was filtered in meta-analyses to investigate the molecular basis of the phenotype observed in the knockout embryos, which exhibited defective hematopoiesis and death. The data from this study are related to the research article “Geminin deletion increases the number of fetal hematopoietic stem cells by affecting the expression of key transcription factors” (Karamitros et al., 2015) [1].The microarray dataset has been deposited at the Gene Expression Omnibus (GEO) under accession GEO: GSE53056. |
| Databáze: | OpenAIRE |
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