Combined vaccination against IL-5 and eotaxin blocks eosinophilia in mice
| Autor: | Gerd Lipowsky, Gary T. Jennings, Manfred Kopf, Ivo Sonderegger, Yu Zou, Nicole Schmitz, Martin F. Bachmann, Marco Landi |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Eotaxin
Chemokine CCL11 Chemokine Ovalbumin medicine.medical_treatment Respiratory Tract Diseases Mice Viral Proteins Eosinophilia medicine Hypersensitivity Animals Interleukin 5 Autoantibodies Allolevivirus Mice Inbred BALB C General Veterinary General Immunology and Microbiology biology business.industry Vaccination Public Health Environmental and Occupational Health Antibody titer respiratory system Allergens respiratory tract diseases Vaccines Virosome Infectious Diseases Cytokine Immunization Immunology biology.protein Molecular Medicine Female medicine.symptom Interleukin-5 business |
| Zdroj: | Vaccine. 28(18) |
| ISSN: | 1873-2518 |
| Popis: | Interleukin-5 (IL-5) is a cytokine which is essential for the maturation of eosinophils in bone marrow and for their release into the blood. Eotaxin is a CC type chemokine implicated in the recruitment of eosinophils in a variety of inflammatory disorders. Since eosinophil-activity is governed by these two pathways, we targeted both IL-5 and eotaxin by active vaccination to block eosinophilia. We produced two vaccines by chemically cross-linking IL-5 or eotaxin to a virus-like particle (VLP) derived from the bacteriophage Qbeta, yielding highly repetitive arrays of these cytokines on the VLP surface. Both vaccines overcame self-tolerance and induced high antibody titers against the corresponding self-molecules in mice. Immunization with either of the two vaccines reduced eosinophilic inflammation of the lung in an ovalbumin (OVA) based mouse model of allergic airway inflammation. Animals immunized with the two vaccines at the same time developed high antibody titers against both cytokines and also reduced eosinophil-infiltration of the lung. These data demonstrate that targeting either IL-5 or eotaxin may lower eosinophilia. Simultaneous immunization against IL-5 and eotaxin demonstrates that such a therapeutic approach may be used to treat complex disorders in which multiple mediators are involved. |
| Databáze: | OpenAIRE |
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