Renal aging change of alpha 1-adrenoceptor in Wistar rats
| Autor: | Shigeharu Kurimoto, Osamu Inagaki, Kozo Hamada, Masahiro Takanashi, Kazuki Kawabe, Nobuo Moriyama |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Vascular wall
Male medicine.medical_specialty Aging Adrenergic receptor Biology Kidney Clonidine Internal medicine Receptors Adrenergic alpha-1 Prazosin medicine Animals Binding site Rats Wistar Adrenergic alpha-Antagonists Pharmacology Binding Sites α1 adrenoceptor Cortex (botany) Rats Kinetics Endocrinology medicine.anatomical_structure Chlorethylclonidine Autoradiography medicine.drug |
| Zdroj: | General pharmacology. 26(2) |
| ISSN: | 0306-3623 |
| Popis: | 1. 1. The aging changes of density of the α 1 -adrenoceptors in the kidney were evaluated with Wistar rats of several ages (8, 52 and 104 weeks old). 2. 2. [ 3 H]prazosin and [ 3 H]YM617 (newly synthesized α 1 -blocker) were used for the ligand. The B max of [ 3 H]prazosin was 74.0 ± 9.5 fmol/mg/protein in 8 week, 52.1 ± 7.3 fmol/mg protein in 52 week, and 31.3 ± 4.2 fmol/mg/protein in 104 week rats, and that of [ 3 H]YM617 was 45.0 ± 6.6 fmol/mg/protein in 8 week, 32.4 ± 5.7 fmol/mg/protein in 52 week, and 19.3 ± 5.5 fmol/mg/protein in 104 week rats. 3. 3. The B max of both ligands for 104 week rats was significantly decreased compared to 8 week rats, however, 52 week rats showed no decrease of B max for both ligands. 4. 4. The K d values showed no difference in these three age groups for both ligands. 5. 5. Autoradiographic study supported the result above mentioned. Furthermore, the binding sites of α 1 -adrenoceptors were mainly in the cortex (vascular wall and peritubular area) and that α 1 -adrenoceptors were chiefly chlorethylclonidine dihydrochloride (CEC) insensitive. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect