Quantitation of hepatitis B surface antigen by an automated chemiluminescent microparticle immunoassay
| Autor: | Yoshinori Iwatani, Isa K. Mushahwar, Kazushige Iinuma, Takahiko Tsuchida, Seishi Asari, Naoko Yamashita, Masanori Kagita, Matsuo Deguchi |
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| Rok vydání: | 2003 |
| Předmět: |
HBsAg
DNA-Directed DNA Polymerase Biology medicine.disease_cause Sensitivity and Specificity law.invention Automation Hepatitis B Chronic Orthohepadnavirus law Virology medicine Humans Hepatitis B e Antigens Chemiluminescence Hepatitis B virus Immunoassay Hepatitis B Surface Antigens medicine.diagnostic_test virus diseases Hepatitis B biology.organism_classification medicine.disease Molecular biology digestive system diseases Microspheres HBeAg Hepadnaviridae Carrier State DNA Viral Luminescent Measurements |
| Zdroj: | Journal of virological methods. 115(2) |
| ISSN: | 0166-0934 |
| Popis: | A fully automated chemiluminescent microparticle immunoassay (Architect HBsAg QT) was used for the detection and quantitation of hepatitis B surface antigen (HBsAg). The assay is capable of processing up to 800 HBsAg tests per hour. The concentration of HBsAg is determined by utilizing a previously generated Architect HBsAg calibration curve. Architect HBsAg QT sensitivity was found to be around 0.2 ng/ml which is equivalent or superior to other known and commercially available enzyme immunoassays and/or chemiluminescent immunoassays. We performed a quantitative study of HBsAg, HBeAg, HBV-DNA and HBV-DNA polymerase in over 733 sera obtained from 43 chronic hepatitis B carriers. Serum HBsAg levels detected by Architect HBsAg QT were found to be higher in HBeAg-positive than in anti-HBe-positive HBV chronic carriers and correlated with the level of serum HBV-DNA and HBV-DNA polymerase. |
| Databáze: | OpenAIRE |
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