Identification of Neuroactive Constituents of the Ethyl Acetate Fraction from Cyperi Rhizoma Using Bioactivity-Guided Fractionation
| Autor: | Dae Sik Jang, Byeol Ryu, Yeomoon Sim, Jeong Hee Kim, Jin Gyu Choi, Myung Sook Oh, Insug Kang, Pil Sung Gu |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Ethyl acetate Fractionation Biochemistry Ferulic acid 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo Drug Discovery Pharmacology Chemistry Cyperi Rhizoma Bioactivity-guided fractionation Neuroprotection In vitro Rhizome Scirpusin B 030104 developmental biology Scirpusin A Molecular Medicine Original Article Luteolin 030217 neurology & neurosurgery Cyperus rotundus |
| Zdroj: | Biomolecules & Therapeutics |
| ISSN: | 1976-9148 2005-4483 |
| DOI: | 10.4062/biomolther.2016.091 |
| Popis: | Cyperi Rhizoma (CR), the rhizome of Cyperus rotundus L., exhibits neuroprotective effects in in vitro and in vivo models of neuronal diseases. Nevertheless, no study has aimed at finding the neuroactive constituent(s) of CR. In this study, we identified active compounds in a CR extract (CRE) using bioactivity-guided fractionation. We first compared the anti-oxidative and neuroprotective activities of four fractions and the CRE total extract. Only the ethyl acetate (EA) fraction revealed strong activity, and further isolation from the bioactive EA fraction yielded nine constituents: scirpusin A (1), scirpusin B (2), luteolin (3), 6′-acetyl-3,6-diferuloylsucrose (4), 4′,6′ diacetyl-3,6-diferuloylsucrose (5), p-coumaric acid (6), ferulic acid (7), pinellic acid (8), and fulgidic acid (9). The activities of constituents 1–9 were assessed in terms of anti-oxidative, neuroprotective, anti-inflammatory, and anti-amyloid-β activities. Constituents 1, 2, and 3 exhibited strong activities; constituents 1 and 2 were characterized for the first time in this study. These results provide evidence for the value of CRE as a source of multi-functional neuroprotectants, and constituents 1 and 2 may represent new candidates for further development in therapeutic use against neurodegenerative diseases. |
| Databáze: | OpenAIRE |
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