Creatine promotes the GABAergic phenotype in human fetal spinal cord cultures

Autor: Uwe Schlattner, Hans Rudolf Widmer, Ekkehard Dreher, Robert H. Andres, Theo Wallimann, Angélique Ducray, Rachel Qualls, Rolf W. Seiler
Přispěvatelé: Laboratoire de bioénergétique fondamentale et appliquée (LBFA), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hamant, Sarah
Jazyk: angličtina
Rok vydání: 2007
Předmět:
MESH: Neurons
Cell Count
MESH: gamma-Aminobutyric Acid
MESH: Spinal Cord
chemistry.chemical_compound
0302 clinical medicine
MESH: Gene Expression Regulation
Developmental
gamma-Aminobutyric Acid
Neurons
MESH: Choline O-Acetyltransferase
0303 health sciences
biology
MESH: Creatine
Kinase
General Neuroscience
Age Factors
Creatine Kinase
MM Form
Gene Expression Regulation
Developmental
Immunohistochemistry
3. Good health
medicine.anatomical_structure
Spinal Cord
MESH: Creatine Kinase
MM Form
GABAergic
medicine.medical_specialty
Central nervous system
Creatine
Choline O-Acetyltransferase
Phosphocreatine
03 medical and health sciences
Fetus
Organ Culture Techniques
Internal medicine
medicine
[SDV.BBM] Life Sciences [q-bio]/Biochemistry
Molecular Biology
Humans
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Molecular Biology
030304 developmental biology
MESH: Age Factors
MESH: Humans
MESH: Cell Count
MESH: Immunohistochemistry
MESH: Fetus
Spinal cord
MESH: Organ Culture Techniques
Endocrinology
chemistry
biology.protein
Creatine kinase
Neurology (clinical)
Neuroscience
030217 neurology & neurosurgery
Developmental Biology
Zdroj: Brain Research
Brain Research, Elsevier, 2007, 1137 (1), pp.50-7. ⟨10.1016/j.brainres.2006.12.038⟩
ISSN: 0006-8993
DOI: 10.1016/j.brainres.2006.12.038⟩
Popis: International audience; In the present study, we investigated the expression pattern of cytosolic brain specific-BB-CK and ubiquitous mitochondrial-creatine kinases (uMt-CK) in developing human spinal cord. Consequently, we studied the effects of creatine treatment on cultured fetal human spinal cord tissue. We found that both CK isoforms were expressed in fetal spinal cord at all time points investigated (5 to 11.5 weeks post conception) and correspondingly specific CK activity was detected. Chronic creatine exposure resulted in significantly higher densities of GABA-immunoreactive neurons in the cultures, while total neuronal cell density was not altered, suggesting a differentiation inducing mechanism of creatine supplementation. Taken together, our observations favour the view that the creatine phosphocreatine system plays an important role in the developing CNS.
Databáze: OpenAIRE
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