Development of a Coflowing Device for the Size-Controlled Preparation of Magnetic-Polymeric Microspheres as Embolization Agents in Magnetic Resonance Navigation Technology
| Autor: | Stoyan Karagiozov, Gilles Soulez, Zeynab Nosrati, Katayoun Saatchi, François Michaud, Ning Li, Sylvain Martel, Sahan A. Ranamukhaarachchi, Urs O. Häfeli |
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| Rok vydání: | 2018 |
| Předmět: |
Materials science
Magnetometer medicine.medical_treatment Biomedical Engineering 02 engineering and technology 030218 nuclear medicine & medical imaging law.invention Biomaterials 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine law Phase (matter) medicine Embolization medicine.diagnostic_test fungi Magnetic resonance imaging 021001 nanoscience & nanotechnology Magnetic field PLGA chemistry Magnetic nanoparticles Particle size 0210 nano-technology Biomedical engineering |
| Zdroj: | ACS Biomaterials Science & Engineering. 4:1092-1102 |
| ISSN: | 2373-9878 |
| DOI: | 10.1021/acsbiomaterials.7b00839 |
| Popis: | Droplet microfluidics technology has recently been introduced to generate particles for many biomedical applications that include therapeutic embolizing agents in hepatic, uterine or bronchial arteries. Embolic agents are available in a variety of shapes and sizes that are adjusted according to the target vessel characteristics. Magnetic embolic agents can additionally be navigated to the target location (e.g., a tumor) through the blood system by applying an external magnetic field. This technology is termed Magnetic Resonance Navigation (MRN). Here we introduce a high throughput method to produce homogeneously sized magnetic microspheres (MMS) as blood vessel embolic agents for use in combination with MRN. The system for MMS production consists of a simple 3D printed micro coflowing device that is able to produce biocompatible, degradation rate controllable poly(lactic-co-glycolic acid) (PLGA) microspheres encasing magnetic nanoparticles. Axisymmetric flow is obtained with a central needle injecting the dispersed phase surrounded by a continuous phase and leads to the formation of size-controlled droplets that turn into homogeneously sized MMS linearly dependent on the inner needle diameter. MMS morphology, mean particle size and size distribution were quantified from SEM images. Magnetic performance of MMS was investigated using a vibrating sample magnetometer. MMS were nontoxic toward HUVEC (human umbilical vein endothelial cells) and HEK293 (human embryonic kidney) cells. The presented micro coflowing method allows for the reliable production of large MMS sized 130-700 μm with narrow size distribution (CV < 7%) and magnetic properties useful for MRN. |
| Databáze: | OpenAIRE |
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