Dynamic electric field assisted multi-dimensional liquid chromatography of biological samples
| Autor: | M. Quaglia, Brian A. Grimes, Dieter Lubda, O. Kornyšova, K.K. Unger, T.P. Hennessy |
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| Rok vydání: | 2005 |
| Předmět: |
Polarity reversal
Analyte Chromatography Chemistry Clinical Biochemistry Size-exclusion chromatography Analytical chemistry Cell Biology General Medicine Reversed-phase chromatography Chromatography Ion Exchange Sensitivity and Specificity Biochemistry Analytical Chemistry Matrix (chemical analysis) Electric field Potential gradient Chromatography Gel Humans Electrophoresis Polyacrylamide Gel Electric potential Blood Chemical Analysis |
| Zdroj: | Journal of Chromatography B. 817:127-137 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2004.10.043 |
| Popis: | Complex biological samples require very high resolution separation strategies. The platform introduced here capitalises on the hyphenation of liquid chromatographic (LC) and electric potential gradient electrochromatographic multi-dimensional separation genres. First-dimension selectivity is provided by simultaneous size exclusion (SEC) and strong cation exchange (SCX) chromatography modes, while the second dimension comprises reversed phase (RP) characteristics in a dynamic (time-variant) electric field. The time-variant potential gradient with reversal of polarity is applied across the second dimension monolithic capillary throughout the duration of the solvent strength gradient elution. Hence, the platform offers comprehensive on-line sample clean-up (matrix depletion, analyte enrichment), fractionation (first dimention LC), and separation (second dimension LC) with the prospect of altering selectivity via polarity reversal dynamic electric field tuning. |
| Databáze: | OpenAIRE |
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