Modeling of cancer metastasis and drug resistance via biomimetic nano-cilia and microfluidics
| Autor: | Ruby Yun-Ju Huang, Andrew M. Wo, Chi-Hao Chang, Hao-Kai Liu, Guan-Syuan Huang, Chi-Ling Chiang, Hsinyu Lee, Ching-Te Kuo, Chiun-Sheng Huang |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Microfluidics
Biophysics Fluorescent Antibody Technique Bioengineering Models Biological Metastasis Biomaterials Tissue culture In vivo Cancer stem cell Biomimetics Cell Line Tumor Neoplasms medicine Humans Cilia Neoplasm Metastasis biology CD44 Spheroid medicine.disease In vitro Cell biology Nanostructures Mechanics of Materials Drug Resistance Neoplasm embryonic structures Ceramics and Composites biology.protein Female Biomedical engineering Chemotaxis assay |
| Zdroj: | Biomaterials. 35(5) |
| ISSN: | 1878-5905 |
| Popis: | Three-dimensional (3D) tissue culture platforms that are capable of mimicking in vivo microenvironments to replicate physiological conditions are vital tools in a wide range of cellular and clinical studies. Here, learning from the nature of cilia in lungs - clearing mucus and pathogens from the airway - we develop a 3D culture approach via flexible and kinetic copolymer-based chains (nano-cilia) for diminishing cell-to-substrate adhesion. Multicellular spheroids or colonies were tested for 3-7 days in a microenvironment consisting of generated cells with properties of putative cancer stem cells (CSCs). The dynamic and reversible regulation of epithelial-mesenchymal transition (EMT) was examined in spheroids passaged and cultured in copolymer-coated dishes. The expression of CSC markers, including CD44, CD133, and ABCG2, and hypoxia signature, HIF-1α, was significantly upregulated compared to that without the nano-cilia. In addition, these spheroids exhibited chemotherapeutic resistance in vitro and acquired enhanced metastatic propensity, as verified from microfluidic chemotaxis assay designed to replicate in vivo-like metastasis. The biomimetic nano-cilia approach and microfluidic device may offer new opportunities to establish a rapid and cost-effective platform for the study of anti-cancer therapeutics and CSCs. |
| Databáze: | OpenAIRE |
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