Nuclear contrast angiography: A simple method for morphological characterization of cerebral arteries
| Autor: | Michael M. Wang, Rushdee Hasan, Yezi Peng, Genggeng Yu, He Meng |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Male
Retinal Artery Cerebral arteries Neovascularization Physiologic Article Brain Ischemia Rats Sprague-Dawley Brain ischemia Mice Cerebral circulation medicine.artery medicine Animals Molecular Biology Cell Size medicine.diagnostic_test business.industry General Neuroscience Endothelial Cells Anatomy Cerebral Arteries medicine.disease Arterial tree Cerebral Angiography Rats Mice Inbred C57BL Middle cerebral artery Angiography Female Neurology (clinical) business Developmental Biology Cerebral angiography Circle of Willis |
| Zdroj: | Brain Research. 1261:75-81 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/j.brainres.2009.01.020 |
| Popis: | Visualization of the cerebral vascular tree is important in experimental stroke and cerebral vascular malformation research. We describe a simple method, nuclear contrast angiography, that enables simultaneous visualization of the arterial tree and cerebral endothelial cells in rodent brain whole mounts. A mixture of latex and black ink was injected into the arterial system of rodents, resulting in high contrast demarcation of the arterial tree of the brain. This method clearly differentiates arteries from veins. We applied this method to demonstrate that 14 days of unilateral carotid artery occlusion induces increases in the caliber of (1) bilateral anterior communicating arteries, (2) bilateral anterior cerebral arteries, and (3) ipsilateral proximal middle cerebral artery of the circle of Willis. Unlike other methods, this procedure selectively stains endothelial nuclei of arteries. Thus, cerebral endothelial nuclei can be visualized, quantitated, and morphologically characterized at the same time the cortical arterial tree is delineated. This method should be useful in studies of stroke and cerebral arteriogenesis, which require the accurate assessment of both arterial diameters and endothelial cell density. |
| Databáze: | OpenAIRE |
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