Stability and association of Smoothened, Costal2 and Fused with Cubitus interruptus are regulated by Hedgehog
| Autor: | Ralph Rodriguez, Laurent Ruel, Armel Gallet, Laurence Lavenant-Staccini, Pascal P. Thérond |
|---|---|
| Přispěvatelé: | Institut de signalisation, biologie du développement et cancer (ISBDC), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA) |
| Rok vydání: | 2003 |
| Předmět: |
MESH: Signal Transduction
Kinesins MESH: Receptors G-Protein-Coupled Receptors G-Protein-Coupled Transduction (genetics) 0302 clinical medicine MESH: Gene Expression Regulation Developmental [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] Drosophila Proteins MESH: Animals Integral membrane protein 0303 health sciences Kinase Gene Expression Regulation Developmental MESH: Transcription Factors Smoothened Receptor Cell biology DNA-Binding Proteins Drosophila melanogaster medicine.anatomical_structure 030220 oncology & carcinogenesis Protein Binding Signal Transduction animal structures Macromolecular Substances MESH: Drosophila Proteins Active Transport Cell Nucleus Embryonic Structures MESH: Active Transport Cell Nucleus Protein Serine-Threonine Kinases Biology MESH: Embryonic Structures MESH: Protein-Serine-Threonine Kinases MESH: Drosophila melanogaster 03 medical and health sciences Mediator medicine Animals MESH: Protein Binding MESH: Transport Vesicles Hedgehog Proteins Transport Vesicles Hedgehog 030304 developmental biology Cell Membrane MESH: Macromolecular Substances MESH: Hedgehog Proteins Cell Biology In vitro MESH: Kinesin Smoothened Nucleus MESH: DNA-Binding Proteins Transcription Factors MESH: Cell Membrane |
| Zdroj: | Nature Cell Biology Nature Cell Biology, Nature Publishing Group, 2003, 5 (10), pp.907-13. ⟨10.1038/ncb1052⟩ |
| ISSN: | 1476-4679 1465-7392 |
| DOI: | 10.1038/ncb1052 |
| Popis: | International audience; The mechanisms involved in transduction of the Hedgehog (Hh) signal are of considerable interest to developmental and cancer biologists. Stabilization of the integral membrane protein Smoothened (Smo) at the plasma membrane is a crucial step in Hh signalling but the molecular events immediately downstream of Smo remain to be elucidated. We have shown previously that the transcriptional mediator Cubitus interruptus (Ci) is associated in a protein complex with at least two other proteins, the kinesin-like Costal2 (Cos2) and the serine-threonine kinase Fused (Fu). This protein complex governs the access of Ci to the nucleus. Here we show that, consequent on the stabilization of Smo, Cos2 and Fu are destabilized. Moreover, we find that the Cos2-Fu-Ci protein complex is associated with Smo in membrane fractions both in vitro and in vivo. We also show that Cos2 binding on Smo is necessary for the Hh-dependent dissociation of Ci from this complex. We propose that the association of the Cos2 protein complex with Smo at the plasma membrane controls the stability of the complex and allows Ci activation, eliciting its nuclear translocation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|