Purinergic activation of a leak potassium current in freshly dissociated myocytes from mouse thoracic aorta
| Autor: | Karima Serir, M. Docquier, Sebastien Hayoz, Jean-Louis Bény, Rostislav Bychkov |
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| Rok vydání: | 2009 |
| Předmět: |
Male
medicine.medical_specialty Indoles Patch-Clamp Techniques Charybdotoxin Physiology Gene Expression Aorta Thoracic Nerve Tissue Proteins Apamin Maleimides Purinergic Agonists Mice chemistry.chemical_compound Adenosine Triphosphate Potassium Channels Tandem Pore Domain Internal medicine Glyburide Potassium Channel Blockers medicine Animals Channel blocker Protein Kinase C Protein kinase C Muscle Cells Reverse Transcriptase Polymerase Chain Reaction Purinergic receptor Hydrogen-Ion Concentration Molecular biology Potassium channel Mice Inbred C57BL Endocrinology chemistry Intracellular |
| Zdroj: | Acta Physiologica. 195:247-258 |
| ISSN: | 1748-1716 1748-1708 |
| DOI: | 10.1111/j.1748-1716.2008.01884.x |
| Popis: | Exogenous ATP elicits a delayed calcium-independent K(+) current on freshly isolated mouse thoracic aorta myocytes. We investigated the receptor, the intracellular pathway and the nature of this current.The patch-clamp technique was used to record ATP-elicited delayed K(+) current in freshly dissociated myocytes.ATP-elicited delayed K(+) current was not inhibited by a 'cocktail' of K(+) channel blockers (4-AP, TEA, apamin, charybdotoxin, glibenclamide). The amplitude of the delayed K(+) current decreased after the reduction of extracellular pH from 7.4 to 6.5. These two characteristics suggest that this current could be carried by the TASK subfamily of 'twin-pore potassium channels' (K2P). Purinergic agonists including dATP, but not ADP, activated the delayed K(+) current, indicating that P2Y(11) is the likely receptor involved in its activation. The PKC activator phorbol ester 12,13-didecanoate stimulated this current. In addition, the PKC inhibitor Gö 6850 partially inhibited it. Real-time quantitative PCR showed that the genes encoding TASK-1 and TASK-2 are expressed.Our results indicate that blocker cocktail-insensitive delayed K(+) current in freshly dissociated aortic myocytes is probably carried by the TASK subfamily of twin-pore channels. |
| Databáze: | OpenAIRE |
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