Interactions of Apolipoproteins AI, AII, B and HDL, LDL, VLDL with Polyurethane and Polyurethane-PEO Surfaces
| Autor: | John L. Brash, R.M. Cornelius, Joseph Macri, K.M. Cornelius |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Very low-density lipoprotein
Apolipoprotein B Surface Properties Lipoproteins Blotting Western Polyurethanes 02 engineering and technology Polyethylene Glycols 03 medical and health sciences chemistry.chemical_compound Adsorption Electrochemistry General Materials Science Spectroscopy 030304 developmental biology Polyurethane High concentration 0303 health sciences Chromatography Ethylene oxide biology Photoelectron Spectroscopy Surfaces and Interfaces 021001 nanoscience & nanotechnology Condensed Matter Physics Biochemistry chemistry biology.protein lipids (amino acids peptides and proteins) Electrophoresis Polyacrylamide Gel 0210 nano-technology Segmented polyurethane Lipoprotein |
| Zdroj: | Langmuir : the ACS journal of surfaces and colloids. 31(44) |
| ISSN: | 1520-5827 |
| Popis: | The lipoproteins (HDL, LDL, VLDL) are important components of blood present in high concentration. Surprisingly, their role in blood-biomaterial interactions has been largely ignored. In previous work apolipoprotein AI (the main protein component of HDL) was identified as a major constituent of protein layers adsorbed from plasma to biomaterials having a wide range of surface properties, and quantitative data on the adsorption of apo AI to a biomedical grade polyurethane were reported. In the present communication quantitative data on the adsorption of apo AI, apo AII and apoB (the latter being a constituent of LDL and VLDL), as well as the lipoprotein particles themselves (HDL, LDL, VLDL), to a biomedical segmented polyurethane (PU) with and without an additive containing poly(ethylene oxide) (material referred to as PEO) are reported. Using radiolabeled apo AI, apo AII, and apoB, adsorption levels on PU from buffer at a protein concentration of 50 μg/mL were found to be 0.34, 0.40, and 0.14 μg/cm(2) (12, 23, and 0.25 nmol/cm(2)) respectively. Adsorption to the PEO surface was |
| Databáze: | OpenAIRE |
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