Comparison of two in vitro methods for the detection of ivermectin resistance in Haemonchus contortus in sheep
| Autor: | Marián Várady, Michal Babják, A. Königová, M. Urda Dolinská |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Medicine (General) biology Resistance (ecology) Agriculture (General) Ecology (disciplines) anthelmintic resistance 030108 mycology & parasitology biology.organism_classification In vitro in vitro detection S1-972 Microbiology ivermectin haemonchus contortus 03 medical and health sciences R5-920 Ivermectin parasitic diseases medicine Animal Science and Zoology Parasitology Haemonchus contortus medicine.drug |
| Zdroj: | Helminthologia, Vol 53, Iss 2, Pp 120-125 (2016) |
| ISSN: | 1336-9083 0440-6605 |
| DOI: | 10.1515/helmin-2015-0002 |
| Popis: | Summary Gastrointestinal parasitic nematodes in sheep cause severe economic losses. Anthelmintics are the most commonly used drugs for prophylaxis and therapy against parasitic helminths. The problem of drug resistance has developed for all commercially available anthelmintics in several genera and classes of helminths. In vitro and in vivo tests are used to detect anthelmintic resistance. Two in vitro methods (larval migration inhibition test and micromotility test) for the detection of ivermectin (IVM) resistance were compared using IVM-resistant and IVM-susceptible isolates of Haemonchus contortus. The degree of resistance for each test was expressed as a resistance factor (RF). The micromotility test was more sensitive for quantitatively measuring the degree of resistance between susceptible and resistant isolates. The RFs for this test for IVM and eprinomectin ranged from 1.00 to 108.05 and from 3.87 to 32.32, respectively. |
| Databáze: | OpenAIRE |
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