Anti-Cancer Activity and Phenolic Content of Extracts Derived from Cypriot Carob (Ceratonia siliqua L.) Pods Using Different Solvents
| Autor: | Haria Hadjipakkou, Constantina P. Kapnissi-Christodoulou, Ioannis J. Stavrou, Alexandru Vasincu, Gregoria Gregoriou, Eftychia Pinakoulaki, Hermann Stuppner, Georgia D Ioannou, Siegfried Aigner, Christiana M. Neophytou, Andreas I. Constantinou, Marios C. Christodoulou, Antonis C. Kakas, Yiota Gregoriou, Atalanti Christou |
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| Rok vydání: | 2021 |
| Předmět: |
Naringenin
DPPH naringenin Ethyl acetate Pharmaceutical Science antiproliferative capacity Analytical Chemistry myricetin chemistry.chemical_compound QD241-441 food Drug Discovery chemoprevention MTT assay Food science Physical and Theoretical Chemistry polyphenols kaempferol carobs Organic Chemistry apoptosis food and beverages food.food LC-MS anti-oxidant activity Ceratonia siliqua chemistry Chemistry (miscellaneous) Polyphenol Molecular Medicine Myricetin Kaempferol |
| Zdroj: | Molecules Volume 26 Issue 16 Molecules, Vol 26, Iss 5017, p 5017 (2021) |
| ISSN: | 1420-3049 |
| Popis: | Extracts derived from the Ceratonia siliqua L. (carob) tree have been widely studied for their ability to prevent many diseases mainly due to the presence of polyphenolic compounds. In this study, we explored, for the first time, the anti-cancer properties of Cypriot carobs. We produced extracts from ripe and unripe whole carobs, pulp and seeds using solvents with different polarities. We measured the ability of the extracts to inhibit proliferation and induce apoptosis in cancer and normal immortalized breast cells, using the MTT assay, cell cycle analysis and Western Blotting. The extracts’ total polyphenol content and anti-oxidant action was evaluated using the Folin–Ciocalteu method and the DPPH assay. Finally, we used LC-MS analysis to identify and quantify polyphenols in the most effective extracts. Our results demonstrate that the anti-proliferative capacity of carob extracts varied with the stage of carob maturity and the extraction solvent. The Diethyl-ether and Ethyl acetate extracts derived from the ripe whole fruit had high Myricetin content and also displayed specific activity against cancer cells. Their mechanism of action involved caspase-dependent and independent apoptosis. Our results indicate that extracts from Cypriot carobs may have potential uses in the development of nutritional supplements and pharmaceuticals. |
| Databáze: | OpenAIRE |
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