A SYBR green I real-time polymerase chain reaction (PCR) assay for detection and quantification of Trichomonas gallinae
| Autor: | Arwid Daugschies, Shahinaz Taha, Tran Nguyen-Ho-Bao, Zaida Rentería-Solís |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Pcr assay
Protozoology - Short Communication Trichomonas Infections Diamines Biology Trichomonas gallinae Real-Time Polymerase Chain Reaction Sensitivity and Specificity 030308 mycology & parasitology Birds 03 medical and health sciences chemistry.chemical_compound Real-time PCR Flagellates In vivo RNA Ribosomal 18S Animals Parasite hosting Benzothiazoles Trichomoniasis Organic Chemicals Gene Phylogeny 030304 developmental biology SYBR green 0303 health sciences General Veterinary Reproducibility of Results General Medicine biology.organism_classification Virology Standard curve Infectious Diseases Real-time polymerase chain reaction chemistry Insect Science Host-Pathogen Interactions Quinolines Trichomonas SYBR Green I Parasitology |
| Zdroj: | Parasitology Research |
| ISSN: | 1432-1955 0932-0113 |
| DOI: | 10.1007/s00436-020-06887-x |
| Popis: | Trichomonas gallinae are parasitic flagellates of importance in wild and domestic birds. The parasite is worldwide distributed, and Columbine birds are its main host. Current research focuses mostly on epidemiological and phylogenetic studies. However, there is still a lack of knowledge regarding parasite-host interaction or therapy development. Real-time PCR is a useful tool for diagnostic and quantification of gene copies in a determined sample. By amplification of a 113-bp region of the 18S small subunit ribosomal RNA gene, a SYBR green-based real-time PCR assay was developed. A standard curve was prepared for quantification analysis. Assay efficiency, linearity, and dissociation analysis were successfully performed. Specificity, sensibility, and reproducibility analysis were tested. This assay could be a useful tool not only for diagnostic purposes but also for future in vivo and in vitro T. gallinae studies. |
| Databáze: | OpenAIRE |
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