Characterization and expression profiling of cucumber kinesin genes during early fruit development: revealing the roles of kinesins in exponential cell production and enlargement in cucumber fruit
| Autor: | Hongjun Yu, Yan Wang, Xue Yong Yang, San Wen Huang, Xiao Ling Liu, Xiao Meng Zhang, Wei Jie Jiang, Guo Qin Liu |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Cell division
Physiology Immunoblotting Arabidopsis Kinesins Plant Science Biology Phragmoplast Genes Plant kinesin expansion chloroplast Microtubule Gene Expression Regulation Plant Plant Cells Botany Cluster Analysis Telophase Cloning Molecular Cell Proliferation Cell Size Plant Proteins Adenosine Triphosphatases plasma membrane Cell growth Gene Expression Profiling Protoplasts food and beverages Gene Expression Regulation Developmental fruit Organ Size Plant cell biology.organism_classification Cell biology phragmoplast Kinesin Cucumis sativus cucumber Research Paper Subcellular Fractions |
| Zdroj: | Journal of Experimental Botany |
| ISSN: | 1460-2431 0022-0957 |
| Popis: | Rapid cell division and expansion in early fruit development are important phases for cucumber fruit yield and quality. Kinesin proteins are microtubule-based motors responsible for modulating cell division and enlargement. In this work, the candidate kinesin genes involved in rapid cell division and expansion during cucumber fruit development were investigated. The morphological and cellular changes during early fruit development were compared in four cucumber genotypes with varied fruit size. The correlation between the expression profiles of cucumber kinesin genes and cellular changes in fruit was investigated. Finally, the biochemical characteristics and subcellular localizations of three candidate kinesins were studied. The results clarified the morphological and cellular changes during early cucumber fruit development. This study found that CsKF2–CsKF6 were positively correlated with rapid cell production; CsKF1 and CsKF7 showed a strongly positive correlation with rapid cell expansion. The results also indicated that CsKF1 localized to the plasma membrane of fast-expanding fruit cells, that CsKF2 might play a role in fruit chloroplast division, and that CsKF3 is involved in the function or formation of phragmoplasts in fruit telophase cells. The results strongly suggest that specific fruit-enriched kinesins are specialized in their functions in rapid cell division and expansion during cucumber fruit development. |
| Databáze: | OpenAIRE |
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