Two-Layer Elastographic 3-D Traction Force Microscopy
| Autor: | Juan C. del Álamo, Ruedi Meili, Begoña Álvarez-González, Shun Zhang, Manuel Gomez-Gonzalez, Richard A. Firtel, Juan C. Lasheras |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microscopy Multidisciplinary Materials science Traction (engineering) Two layer Atomic Force Bioengineering Mechanics Microscopy Atomic Force Poisson distribution Traction force microscopy Article Highly sensitive Imaging Physarum 03 medical and health sciences symbols.namesake Imaging Three-Dimensional 030104 developmental biology Experimental proof Traction Self-healing hydrogels Three-Dimensional symbols |
| Zdroj: | Scientific reports, vol 7, iss 1 Scientific Reports |
| Popis: | Cellular traction force microscopy (TFM) requires knowledge of the mechanical properties of the substratum where the cells adhere to calculate cell-generated forces from measurements of substratum deformation. Polymer-based hydrogels are broadly used for TFM due to their linearly elastic behavior in the range of measured deformations. However, the calculated stresses, particularly their spatial patterns, can be highly sensitive to the substratum’s Poisson’s ratio. We present two-layer elastographic TFM (2LETFM), a method that allows for simultaneously measuring the Poisson’s ratio of the substratum while also determining the cell-generated forces. The new method exploits the analytical solution of the elastostatic equation and deformation measurements from two layers of the substratum. We perform an in silico analysis of 2LETFM concluding that this technique is robust with respect to TFM experimental parameters, and remains accurate even for noisy measurement data. We also provide experimental proof of principle of 2LETFM by simultaneously measuring the stresses exerted by migrating Physarum amoeboae on the surface of polyacrylamide substrata, and the Poisson’s ratio of the substrata. The 2LETFM method could be generalized to concurrently determine the mechanical properties and cell-generated forces in more physiologically relevant extracellular environments, opening new possibilities to study cell-matrix interactions. |
| Databáze: | OpenAIRE |
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