Prepubertal exposure to low doses of sodium arsenite impairs spermatogenesis and epididymal histophysiology in rats
| Autor: | Ricardo Rodrigues Samelo, Ana Priscila Gomes Silva, Fabio A. Duarte, Ítalo Braga Castro, Marcella da Silva Araujo Santiago, Paloma da Cunha de Medeiros, Juliana Elaine Perobelli |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
Sodium arsenite Arsenites Health Toxicology and Mutagenesis Population 010501 environmental sciences Management Monitoring Policy and Law Biology Toxicology 01 natural sciences Andrology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Arsenic Poisoning Testis Toxicity Tests medicine Animals Sexual Maturation Rats Wistar Spermatogenesis education 0105 earth and related environmental sciences Epididymis education.field_of_study Reproduction Organ Size General Medicine Sodium Compounds Spermatozoa Sperm Rats Fertility medicine.anatomical_structure chemistry Vacuolization 030220 oncology & carcinogenesis Toxicity Reproductive toxicity Water Pollutants Chemical |
| Zdroj: | Environmental Toxicology. 34:83-91 |
| ISSN: | 1522-7278 1520-4081 |
| DOI: | 10.1002/tox.22660 |
| Popis: | For the first time, juvenile toxicity of inorganic arsenic (As) was investigated in male rats, focusing on reproductive effects. As is a metalloid naturally occurring in the environment, being the inorganic forms the most toxics. Contaminated drinking water and agricultural products are the main prospectors of intoxication for general population. In the present study, Wistar male rats (21 days old) were distributed into three groups (n = 10/group): control (received vehicle-filtered drinking water), As1 (received AsNaO2 at 0.01 mg L-1 ) and As2 (received AsNaO2 at 10 mg L-1 ). The animals were euthanized on PND 53. Testicular damages increased in As1 and As2 compared to control (ie, presence of vacuolization, acidophilic cells, and epithelium degeneration). Testicular interstitium of As1 and As2 presented fluid's increase and intense inflammatory infiltration. In the epididymis there was reduction of sperm amount in the lumen, besides epithelium areas presenting cribriform aspect in As1 and As2, exfoliation of cells in the light (in As1) and vacuoles (in As2). In epididymis interstitium, inflammatory infiltrates were observed in initial segment of As1 and As2. AsNaO2 changed immunolabeling pattern for androgen receptor in epididymis of As2, although serum testosterone levels was statistically comparable to control. Mass spectrometry revealed higher As concentrations in testis and epididymis of As2 compared to As1 and Control. These results indicate compromise of spermatogenesis and epididymal histophysiology in AsNaO2 -treated animals, possibly impairing sperm quality and fertility in long-term, even at low levels of exposure. Investigations about the reversibility of reproductive damages are necessary to better understand the mechanisms of As reproductive toxicity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text