A Novel Acyl-CoA Synthetase, ACS5, Expressed in Intestinal Epithelial Cells and Proliferating Preadipocytes
| Autor: | Man Jong Kang, Hiroaki Iijima, Eisaku Oikawa, Hironobu Sasano, Takashi Suzuki, Akihisa Kamataki, Hiroyuki Sato, Tokuo T. Yamamoto, Hiroshi Nagura, Takahiro Fujino, Hiroyuki Suzuki |
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| Rok vydání: | 1998 |
| Předmět: |
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Complementary Long-chain-fatty-acid—CoA ligase Molecular Sequence Data Adipose tissue Ileum In situ hybridization ACSL5 Biochemistry Mitochondrial Proteins Coenzyme A Ligases Adipocytes medicine Animals Amino Acid Sequence RNA Messenger Northern blot Molecular Biology In Situ Hybridization Base Sequence Chemistry Fatty Acids Epithelial Cells General Medicine Blotting Northern Dietary Fats Molecular biology Small intestine Rats Intestines medicine.anatomical_structure Gene Expression Regulation Lipogenesis Cell Division |
| Zdroj: | Journal of Biochemistry. 124:679-685 |
| ISSN: | 0021-924X |
| DOI: | 10.1093/oxfordjournals.jbchem.a022165 |
| Popis: | We report here the identification, characterization, and expression of a novel rat acyl-CoA synthetase (ACS) designated as ACS5. ACS5 consists of 683 amino acids and is approximately 60% identical to the previously characterized ACS1 and ACS2. ACS5 was overproduced in Escherichia coli cells and then purified to near homogeneity. The purified enzyme utilized a wide range of saturated fatty acids similar to those utilized by ACS1 and ACS2, but differed in its preference for C16-C18 unsaturated fatty acids. Northern blot analysis revealed that ACS5 mRNA is present most abundantly in the small intestine, and to a much lesser extent in the lung, liver, adrenal gland, adipose tissue, and kidney. In situ hybridization of rat ileum revealed abundant accumulation of ACS5 transcripts in foveolar epithelial cells. The hepatic level of ACS5 mRNA was significantly increased by refeeding a fat-free high sucrose diet and reduced by fasting or refeeding a high cholesterol diet, whereas that in the small intestine was not significantly altered by various dietary conditions. In contrast to the absence of ACS1 mRNA in undifferentiated 3T3-L1 preadipocytes, ACS5 mRNA was present in proliferating 3T3-L1 preadipocytes and its level remained unaltered during differentiation, suggesting that ACS5 may provide the acyl-CoA utilized for the synthesis of cellular lipids in proliferating preadipocytes. |
| Databáze: | OpenAIRE |
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