In vitro osteoinduction of human mesenchymal stem cells in biomimetic surface modified titanium alloy implants
| Autor: | María Ángeles Pérez, Sonia Santander, Clara Alcaine, Clementina Rodellar, Ignacio Ochoa, Manuel Doblaré, Jaber Lyahyai |
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| Rok vydání: | 2014 |
| Předmět: |
Calcium Phosphates
Materials science Surface Properties Cell Culture Techniques chemistry.chemical_element Core Binding Factor Alpha 1 Subunit Real-Time Polymerase Chain Reaction Osseointegration Coated Materials Biocompatible Biomimetic Materials Osteogenesis Biomimetics Aluminum Oxide Alloys Cell Adhesion Humans Cell adhesion Cell Shape General Dentistry Cells Cultured Cell Proliferation Dental Implants Titanium Osteoblasts Photoelectron Spectroscopy Mesenchymal stem cell Titanium alloy Cell Differentiation Mesenchymal Stem Cells Adhesion Electrochemical Techniques Titanium oxide chemistry Microscopy Electron Scanning Ceramics and Composites Surface modification Osteopontin Porosity Biomedical engineering Dental Alloys |
| Zdroj: | Dental materials journal. 33(3) |
| ISSN: | 1881-1361 |
| Popis: | Interaction between cells and implant surface is crucial for clinical success. This interaction and the associated surface treatment are essential for achieving a fast osseointegration process. Several studies of different topographical or chemical surface modifications have been proposed previously in literature. The Biomimetic Advanced Surface (BAS) topography is a combination of a shot blasting and anodizing procedure. Macroroughness, microporosity of titanium oxide and Calcium/Phosphate ion deposition is obtained. Human mesenchymal stem cells (hMCSs) response in vitro to this treatment has been evaluated. The results obtained show an improved adhesion capacity and a higher proliferation rate when hMSCs are cultured on treated surfaces. This biomimetic modification of the titanium surface induces the expression of osteblastic differentiation markers (RUNX2 and Osteopontin) in the absence of any externally provided differentiation factor. As a main conclusion, our biomimetic surface modification could lead to a substantial improvement in osteoinduction in titanium alloy implants. |
| Databáze: | OpenAIRE |
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