Increased mononuclear cell membrane fluidity and increased B lymphocyte HLA class I expression in IgA nephropathy
| Autor: | T. H. Thomas, T. R. Ringrose, R. Wilkinson |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Adult
medicine.medical_specialty Membrane Fluidity Lymphocyte Human leukocyte antigen Peripheral blood mononuclear cell Nephropathy Blood cell chemistry.chemical_compound Internal medicine Membrane fluidity Humans Medicine Cells Cultured B-Lymphocytes Transplantation Creatinine business.industry Histocompatibility Antigens Class I Glomerulonephritis IGA Glomerulonephritis Middle Aged medicine.disease Endocrinology medicine.anatomical_structure chemistry Nephrology Immunology Leukocytes Mononuclear business |
| Zdroj: | Nephrology Dialysis Transplantation. 11:287-292 |
| ISSN: | 1460-2385 0931-0509 |
| Popis: | Background. The functions of membrane bound proteins are regulated by the physical properties of the cell membrane. Lymphocyte dysfunction in IgA nephropathy may therefore be related to abnormal cell membrane fluidity. In this study we have examined peripheral blood mononuclear cell membrane fluidity and the expression of HLA antigens on the surface of lymphocytes in IgA nephropathy subjects compared to normal controls and disease controls. Methods. Twenty-two IgA nephropathy subjects with normal or mildly elevated serum creatinine (serum creatinine < 130 μmol/l), 21 normal control subjects, and 16 disease control subjects were studied. Fluorescence anisotropy was determined using diphenylhexatriene, which partitions deep into the cell membrane, and trimethylammonium-DPH, which partitions more superficially. The lymphocyte expression of HLA antigens was measured by flow cytometry. Results. Fluorescence anisotropy for diphenylhexatriene of mononuclear cells from IgA nephropathy subjects was significantly lower compared to mononuclear cells from normal control subjects and disease control subjects, indicating higher membrane fluidity (median values, IgA nephropathy 0.161 ; normal control 0.175 ; disease control 0.175 ; P |
| Databáze: | OpenAIRE |
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