Sensitive Rapid Fluorescence Polarization Immunoassay for Free Mycophenolic Acid Determination in Human Serum and Plasma
| Autor: | Ana B. Descalzo, Bettina Glahn-Martínez, Francesca Salis, Guillermo Orellana, María C. Moreno-Bondi, Elena Benito-Peña |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
Chromatography Molecular Structure Chemistry Infrared Rays 010401 analytical chemistry 02 engineering and technology Plasma Mycophenolic Acid 021001 nanoscience & nanotechnology 01 natural sciences Fluorescence Mycophenolic acid 0104 chemical sciences Analytical Chemistry Fluorescence Polarization Immunoassay medicine Fluorescence polarization immunoassay Humans Female 0210 nano-technology medicine.drug Fluorescent Dyes |
| Zdroj: | Analytical chemistry. 90(8) |
| ISSN: | 1520-6882 |
| Popis: | In this Article, we describe a fluorescence polarization immunoassay (FPIA) using a new label-near-infrared fluorescent dye. The developed FPIA method was optimized for the rapid analysis of free mycophenolic acid (MPA) in plasma of transplanted patients. The approach is based on the fluorescence competitive assay between the target immunosuppressant and a novel emissive near-infrared fluorescent dye-tagged MPA and MPA-AO for the binding sites of the anti-MPA antibody. The fluorescent analogue of MPA exhibits emission at 654 nm upon excitation at 629 nm (λ |
| Databáze: | OpenAIRE |
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