Viewing of Complex Molecules of Ethidium Bromide and Plasmid DNA in Solution by Atomic Force Microscopy
| Autor: | Masamichi Tsuboi, Shunji Katsumata, Toschitake Iwamoto, Kuniharu Utsuno |
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| Rok vydání: | 2001 |
| Předmět: |
Aqueous solution
Spectrophotometry Infrared Chemistry Stereochemistry Intercalation (chemistry) DNA General Chemistry General Medicine Microscopy Atomic Force PBR322 Solutions Molecular dynamics chemistry.chemical_compound Crystallography Ethidium Drug Discovery Nucleic Acid Conformation DNA supercoil Molecule Electrophoresis Polyacrylamide Gel Ethidium bromide Plasmids |
| Zdroj: | Chemical and Pharmaceutical Bulletin. 49:413-417 |
| ISSN: | 1347-5223 0009-2363 |
| DOI: | 10.1248/cpb.49.413 |
| Popis: | Tertiary structure changes in plasmid DNA, induced by ethidium bromide intercalation, have been observed in aqueous solutions by the use of an atomic force microscope. A relaxed closed circular pBR322 molecule became a positively supercoiled complex on the drug binding. The supercoiling always resulted in an interwound (or a plectonemic) form, but never a solenoidal (or a toroidal) form. A quantitative analysis of the compactness of such supercoiled complexes has been carried out. |
| Databáze: | OpenAIRE |
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