In vitro toxicity of serum protein-adsorbed citrate-reduced gold nanoparticles in human lung adenocarcinoma cells
| Autor: | Kwang Su Ock, Soo Hwa Jang, Jin Park, Sang-Woo Joo, Saeromi Jeong, Seon Young Choi, So Yeong Lee, Jin-Ho Park |
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| Rok vydání: | 2011 |
| Předmět: |
Lung Neoplasms
Cell Survival Metal Nanoparticles Adenocarcinoma of Lung Apoptosis Adenocarcinoma Toxicology Citric Acid chemistry.chemical_compound Dynamic light scattering Microscopy Electron Transmission Lactate dehydrogenase Cell Line Tumor Zeta potential Animals Humans Cytotoxicity A549 cell Chemistry General Medicine Blood Proteins Fetal Blood Blood proteins Biochemistry Colloidal gold Biophysics Cattle Adsorption Gold Oxidation-Reduction Fetal bovine serum |
| Zdroj: | Toxicology in vitro : an international journal published in association with BIBRA. 26(2) |
| ISSN: | 1879-3177 |
| Popis: | We examined the cytotoxicity effect of the serum protein coated gold nanoparticles (AuNPs) in the A549 cells. Negatively charged AuNPs were prepared by chemical reduction using citrate. The dimension and surface charge of AuNPs were characterized using transmission electron microscopy (TEM), dynamic light scattering (DLS), and zeta potential measurements. The AuNPs modified by the citrate anion were presumed to adsorb the serum proteins as indicated from the visible absorption spectroscopy, DLS, and quartz crystal microbalance (QCM) data. The QCM results indicated that among the constituents, fetal bovine serum (FBS) should be the major adsorbate species on the AuNPs incubated in the RPMI medium. The internalization of AuNPs into the A549 cells was also monitored using TEM and dark-field microscopy (DFM). Both methylthiazol tetrazolium (MTT) and lactate dehydrogenase (LDH) assays revealed that AuNPs were toxic as determined by their half-maximal inhibitory concentration. A flow cytometric and real-time PCR analysis of apoptotic genes along with the ATP depletion measurements suggested that AuNPs induce cell damages through extrinsic and intrinsic apoptotic pathways. |
| Databáze: | OpenAIRE |
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