The nervous system as ectopic germinal center: CXCL13 and IgG in lyme neuroborreliosis
| Autor: | Libin Li, Andrew R. Pachner, Kavitha Narayan, Sheela Amrute, Diego Cadavid, Donna Dail, Patricia Fitzgerald-Bocarsly |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Chemokine Biology Peripheral blood mononuclear cell Nervous System Cell Line Lyme disease medicine Animals Humans Lyme Neuroborreliosis CXCL13 Borrelia burgdorferi Immune response gene Reverse Transcriptase Polymerase Chain Reaction Germinal center medicine.disease biology.organism_classification Flow Cytometry Germinal Center Chemokine CXCL13 Immunohistochemistry Macaca mulatta Neurology Immunoglobulin G Immunology biology.protein Leukocytes Mononuclear Neurology (clinical) Chemokines CXC |
| Zdroj: | Annals of neurology. 57(6) |
| ISSN: | 0364-5134 |
| Popis: | Lyme neuroborreliosis (LNB) is a chronic infection in which B-cell activation, plasma cell infiltration, and enhanced Ig production in infected tissue are prominent feature. However, little is known about how B cells and plasma cells invade and persist in target organs. To assess this issue, we developed real-time PCR measurements of IgG and CXCL13 production. We used these RNA assays and specific enzyme-linked immunosorbent assays for protein and demonstrated that human peripheral blood mononuclear cells (PBMCs), stimulated by Borrelia burgdorferi sonicate, produced CXCL13 and IgG. Magnetic separation of PBMC populations and flow cytometry showed that CXCL13 is produced by dendritic cells. We then measure the expression of CXCL13 and IgG in tissues and correlated the expression of these host genes with spirochetal load. We also measured expression of dbpA and BBK32, two spirochetal genes important in chronic infection. There was a strong correlation between host immune response gene expression (CXCL13 and IgG) and spirochetal load. Immunohistochemistry of infected nonhuman primates tissue confirmed that CXCL13 is expressed in the nervous system. We conclude that persistent production of CXCL13 and IgG within infected tissue, two characteristics of ectopic germinal centers, are definitive features of LNB. |
| Databáze: | OpenAIRE |
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