Association of circulatory asymmetric dimethylarginine (ADMA) with diabetic nephropathy in Asian Indians and its causative role in renal cell injury
| Autor: | Prabu Paramasivam, Saravanakumar Sundararajan, Nagaraj Manickam, Muthuswamy Balasubramanyam, Shanthirani C. Subramanian, Balachandar Venkatesan, Isaivani Jayachandran, Viswanathan Mohan |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male medicine.medical_specialty Clinical Biochemistry India 030204 cardiovascular system & hematology Arginine Cell Line Diabetic nephropathy 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Asian People Internal medicine Diabetes mellitus medicine Renal fibrosis Albuminuria Animals Humans Diabetic Nephropathies Glycated Hemoglobin Kidney Creatinine business.industry Type 2 Diabetes Mellitus General Medicine Fibroblasts Middle Aged medicine.disease Glomerular Mesangium Rats 030104 developmental biology Endocrinology medicine.anatomical_structure chemistry Diabetes Mellitus Type 2 Female medicine.symptom business Asymmetric dimethylarginine Biomarkers |
| Zdroj: | Clinical biochemistry. 50(15) |
| ISSN: | 1873-2933 |
| Popis: | Aim Asymmetric dimethylarginine (ADMA) is involved in the regulation of nitric oxide synthesis and in the maintenance of vascular tone and structure. But the role and status of ADMA in diabetes induced kidney injury is not clear. Hence this study is investigating the role of ADMA in the progression of kidney injury and its circulatory status in Asian Indians with and without diabetic nephropathy. Methods Recruited study subjects were divided into normal glucose tolerance (NGT), type 2 diabetes mellitus (T2DM) and T2DM with micro or macroalbuminuria. Albuminuria was calculated using urinary albumin and creatinine ratio (UACR). ADMA was measured using ELISA. Kidney cell damage in terms of fibrotic markers and ADMA metabolism in terms of DDAH activity were investigated in kidney fibroblasts and mesangial cells. Results There was a significant elevation in plasma ADMA levels in micro and macroalbuminuric diabetic patients. We found a significant positive correlation between ADMA and UACR, serum creatinine, HbA1C and fasting plasma glucose. A cut-off value of ADMA, 0.666 μM/l had a sensitivity and specificity of 70.0% and 65.6%, respectively for detecting diabetic nephropathy. DDAH activity was significantly decreased and fibrotic markers such as fibronectin and α-SMA were significantly increased upon high glucose and ADMA treatment. Conclusion We are suggesting a causative role of ADMA in the development of kidney injury in terms of renal fibrosis and also a cut point of 0.666 μM/l of plasma ADMA level appears to be a predictive risk threshold for diabetic nephropathy in south Asian Indian population. |
| Databáze: | OpenAIRE |
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