Elucidation of novel budding yeast separase mutants
| Autor: | Takashi Ushimaru, Naoki Koike, Muhammad Waliullah Talukdar, Akter Mst Yeasmin, Masayoshi Nagai, Yoshihito Shimizu |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
DNA repair Saccharomyces cerevisiae Green Fluorescent Proteins Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry 03 medical and health sciences Bleomycin Osmotic Pressure Sister chromatids Molecular Biology Mitosis Separase Anaphase Genetics biology Cohesin Organic Chemistry Temperature General Medicine biology.organism_classification Cell biology 030104 developmental biology Securin Mutation Benomyl Biotechnology |
| DOI: | 10.6084/m9.figshare.1591144 |
| Popis: | The mitotic separase cleaves Scc1 in cohesin to allow sister chromatids to separate from each other upon anaphase onset. Separase is also required for DNA damage repair. Here, we isolated and characterized 10 temperature-sensitive (ts) mutants of separase ESP1 in the budding yeast Saccharomyces cerevisiae. All mutants were defective in sister chromatid separation at the restricted temperature. Some esp1-ts mutants were hypersensitive to the microtubule poison benomyl and/or the DNA-damaging agent bleomycin. Overexpression of securin alleviated the growth defect in some esp1-ts mutants, whereas it rather exacerbated it in others. The Drosophila Pumilio homolog MPT5 was isolated as a high-dosage suppressor of esp1-ts cells. We discuss various features of separase based on these findings. Securin positively and negatively regulates separase function. In addition, extracellular inputs affect separase function. |
| Databáze: | OpenAIRE |
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