Identification of bacterial biofilm and the Staphylococcus aureus derived protease, staphopain, on the skin surface of patients with atopic dermatitis
| Autor: | Jan Potempa, Kornelia Przybyszewska, Sigrid Eriksson, Artur Schmidtchen, Andreas Sonesson, Julia R. Davies, Sven Kjellström, Matthias Mörgelin |
|---|---|
| Přispěvatelé: | Lee Kong Chian School of Medicine (LKCMedicine) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Adult
0301 basic medicine Staphylococcus aureus skin Medicin och hälsovetenskap medicine.medical_treatment Science Microbial Sensitivity Tests Biology medicine.disease_cause Medical and Health Sciences Article Dermatitis Atopic Microbiology 03 medical and health sciences Cathelicidins In vivo medicine Humans Staphopain Amino Acid Sequence Skin Multidisciplinary Protease Biofilm Atopic dermatitis biochemical phenomena metabolism and nutrition Antimicrobial medicine.disease biology.organism_classification Peptide Fragments infection Anti-Bacterial Agents Skin diseases Cysteine Endopeptidases 030104 developmental biology Biofilms Medicine Bacterial infection Bacteria Antimicrobial Cationic Peptides |
| Zdroj: | Scientific Reports, Vol 7, Iss 1, Pp 1-12 (2017) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by an impaired epidermal barrier, dysregulation of innate and adaptive immunity, and a high susceptibility to bacterial colonization and infection. In the present study, bacterial biofilm was visualized by electron microscopy at the surface of AD skin. Correspondingly, Staphylococcus aureus (S. aureus) isolates from lesional skin of patients with AD, produced a substantial amount of biofilm in vitro. S. aureus biofilms showed less susceptibility to killing by the antimicrobial peptide LL-37 when compared with results obtained using planktonic cells. Confocal microscopy analysis showed that LL-37 binds to the S. aureus biofilms. Immuno-gold staining of S. aureus biofilm of AD skin detected the S. aureus derived protease staphopain adjacent to the bacteria. In vitro, staphopain B degraded LL-37 into shorter peptide fragments. Further, LL-37 significantly inhibited S. aureus biofilm formation, but no such effects were observed for the degradation products. The data presented here provide novel information on staphopains present in S. aureus biofilms in vivo, and illustrate the complex interplay between biofilm and LL-37 in skin of AD patients, possibly leading to a disturbed host defense, which facilitates bacterial persistence. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |