Human sperm Toll-like receptor 4 (TLR4) mediates acrosome reaction, oxidative stress markers, and sperm parameters in response to bacterial lipopolysaccharide in infertile men

Autor: Hammadi Attia, Afifa Sellami, Tarek Rebai, Saloua Lassoued, Malek Mseddi, Nozha Chakroun, Sana Sahnoun
Rok vydání: 2017
Předmět:
Adult
Lipopolysaccharides
Male
0301 basic medicine
endocrine system
medicine.medical_specialty
Acrosome reaction
Semen
Semen analysis
medicine.disease_cause
Male infertility
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Gamete Biology
Malondialdehyde
Internal medicine
Genetics
medicine
Humans
Infertility
Male
reproductive and urinary physiology
Genetics (clinical)
Sperm motility
030219 obstetrics & reproductive medicine
medicine.diagnostic_test
urogenital system
Chemistry
Acrosome Reaction
Obstetrics and Gynecology
General Medicine
Middle Aged
medicine.disease
Spermatozoa
Sperm
Semen Analysis
Toll-Like Receptor 4
Oxidative Stress
Fertility
030104 developmental biology
Endocrinology
Reproductive Medicine
Sperm Motility
Biomarkers
Oxidative stress
Developmental Biology
Zdroj: Journal of Assisted Reproduction and Genetics. 34:1067-1077
ISSN: 1573-7330
1058-0468
Popis: To study the role of Toll-like receptor 4 (TLR4) in human spermatozoa and to assess sperm parameters, oxidative stress markers, and acrosome reaction in response to the stimulation of TLR4 by its ligand, the lipopolysaccharide (LPS), as a major endotoxin of Gram-negative bacteria. Our study was carried out in 73 sperm samples from patients undergoing semen analysis for couple infertility investigations. The studied patients were divided into three groups: normozoospermic fertile patients (n = 13), patients with abnormal and leukospermic semen (n = 13), and patients with abnormal and non-leukospermic semen (n = 47). TLR4 expression in human spermatozoa was initially analyzed by western blot. Sperm samples were incubated in the presence of LPS (200 ng/ml) for 18 h. Then, sperm motility and vitality were evaluated by microscopic observation and oxidative stress markers as malondialdehyde (MDA) and carbonyl groups (CG) were spectrophotometrically assessed in neat and selected sperm. A triple-stain technique was also performed to evaluate acrosome reaction in 15 sperm samples from infertile patients. TLR4 expression was confirmed in human spermatozoa with a molecular weight of 69 kDa. In the normozoospermic group, no significant differences in sperm parameters and oxidative stress markers were shown after incubation with LPS in neat and selected sperms. Regarding samples from the non-leukospermic group, LPS reduced spermatozoa motility and vitality rates in selected sperm (P = 0.003; P = 0.004, respectively). A significant increase of MDA and CG levels was also detected (P = 0.01; P = 0.02, respectively). However, only the MDA levels were significantly increased (P = 0.01) in neat LPS-stimulated sperm. The same results were shown within the leukospermic group. The comparison between the two groups, leukospermic and non-leukospermic, in selected sperms showed a more important LPS effect in the leukospermic group significantly on motility and MDA rates (P = 0.006; P = 0.009, respectively). Furthermore, a significant decrease in reacted spermatozoa rate was detected in response to LPS in selected sperm samples from infertile men (P = 0.03). These findings indicate that human spermatozoa express TLR4 and respond to LPS stimulation with alterations in viability, motility, and the acrosome reaction implicating reactive oxygen species (ROS) production in sperm samples from infertile patients.
Databáze: OpenAIRE
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