Aerosol-Jet-Printed Graphene Immunosensor for Label-Free Cytokine Monitoring in Serum
| Autor: | Ethan B. Secor, Emily A. Smith, Kshama Parate, Jonathan C. Claussen, Mark C. Hersam, Jesse M. Hostetter, Dapeng Jing, Shaowei Ding, Sonal V. Rangnekar, Deyny Mendivelso-Perez |
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| Rok vydání: | 2020 |
| Předmět: |
Materials science
Biocompatibility Polymers Surface Properties Nanotechnology Biosensing Techniques 02 engineering and technology Imides Microscopy Atomic Force Spectrum Analysis Raman 010402 general chemistry Electrochemistry 01 natural sciences Signal Antibodies law.invention Interferon-gamma Limit of Detection law Animals General Materials Science Electrodes Aerosols Detection limit Microscopy Confocal Inkwell Graphene Spectrum Analysis Electric Conductivity Collodion Electrochemical Techniques Carbon Dioxide 021001 nanoscience & nanotechnology Interleukin-10 Nanostructures 0104 chemical sciences Printed electronics Printing Three-Dimensional Cattle Graphite Ink Reactive Oxygen Species 0210 nano-technology Biosensor |
| Zdroj: | ACS Applied Materials & Interfaces. 12:8592-8603 |
| ISSN: | 1944-8252 1944-8244 |
| DOI: | 10.1021/acsami.9b22183 |
| Popis: | Graphene-based inks are becoming increasingly attractive for printing low-cost and flexible electrical circuits due to their high electrical conductivity, biocompatibility, and manufacturing scalability. Conventional graphene printing techniques, such as screen and inkjet printing, are limited by stringent ink viscosity requirements properties and large as-printed line width that impedes the performance of printed biosensors. Here, we report an aerosol-jet-printed (AJP) graphene-based immunosensor capable of monitoring two distinct cytokines: interferon gamma (IFN-γ) and interleukin 10 (IL-10). Interdigitated electrodes (IDEs) with 40 μm finger widths were printed from graphene-nitrocellulose ink on a polyimide substrate. The IDEs were annealed in CO2 to introduce reactive oxygen species on the graphene surface that act as chemical handles to covalently link IFN-γ and IL-10 antibodies to the graphene surfaces. The resultant AJP electrochemical immunosensors are capable of monitoring cytokines in serum with wide sensing range (IFN-γ: 0.1-5 ng/mL; IL-10: 0.1-2 ng/mL), low detection limit (IFN-γ: 25 pg/ml and IL-10: 46 pg/ml) and high selectivity (antibodies exhibited minimal cross-reactivity with each other and IL-6) without the need for sample prelabeling or preconcentration. Moreover, these biosensors are mechanically flexible with minimal change in signal output after 250 bending cycles over a high curvature (Φ = 5 mm). Hence, this technology could be applied to numerous electrochemical applications that require low-cost electroactive circuits that are disposable and/or flexible. |
| Databáze: | OpenAIRE |
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