Molecular cloning and physical mapping of the Neurospora crassa 74-OR23-1A mitochondrial genome
| Autor: | Beverly D. Smolich, John W. Taylor |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Genetics
Neurospora crassa biology Genes Fungal Nucleotide Mapping EcoRI DNA Restriction Enzymes General Medicine Molecular cloning biology.organism_classification DNA Mitochondrial Genome Restriction fragment Neurospora Restriction enzyme Restriction site Restriction map biology.protein Cloning Molecular |
| Zdroj: | Current Genetics. 9:597-603 |
| ISSN: | 1432-0983 0172-8083 |
| DOI: | 10.1007/bf00381173 |
| Popis: | To provide for thorough sampling of the Neurospora crassa mitochondrial genome for evolutionary studies, recombinant plasmids containing each of the EcoRI digestion fragments of the genome were assembled and used to map the locations of 89 additional restriction endonuclease cleavage sites, representing 10 newly mapped enzymes and 2 previously unmapped HincII sites. Data used to locate new restriction sites were obtained from digestions of whole mitochondrial DNA, digestions of the cloned EcoRI mitochondrial DNA fragments and hybridizations between new restriction fragments and the cloned fragments. Length measurements of the total genome and of EcoRI fragment 1 are larger than commonly reported. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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