Detection and enumeration of airborne biocontaminants
| Autor: | Mark P. Buttner, Patricia Cruz, Linda D. Stetzenbach |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Aerosols
Microbiological Techniques Bacteriological Techniques Bacteria Air Air microbiology Air Microbiology Biomedical Engineering Bioengineering Biology Contamination Polymerase Chain Reaction RNA Ribosomal Air Pollution Indoor Environmental chemistry Environmental Microbiology Enumeration Biochemical engineering Filtration Biotechnology |
| Zdroj: | Current Opinion in Biotechnology. 15:170-174 |
| ISSN: | 0958-1669 |
| DOI: | 10.1016/j.copbio.2004.04.009 |
| Popis: | The sampling and analysis of airborne microorganisms has received attention in recent years owing to concerns with mold contamination in indoor environments and the threat of bioterrorism. Traditionally, the detection and enumeration of airborne microorganisms has been conducted using light microscopy and/or culture-based methods; however, these analyses are time-consuming, laborious, subjective and lack sensitivity and specificity. The use of molecular methods, such as quantitative polymerase chain reaction amplification, can enhance monitoring strategies by increasing sensitivity and specificity, while decreasing the time required for analysis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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