Cerebrospinal fluid neopterin as marker of the meningo-encephalitic stage of Trypanosoma brucei gambiense sleeping sickness
| Autor: | Xavier Robin, Krister Kristensson, Sanjeev Krishna, Enock Matovu, Dieudonné Mumba Ngoyi, Jean-Charles Sanchez, Sylvie Bisser, Veerle Lejon, Joseph Mathu Ndung'u, Bertrand Courtioux, Natacha Turck, Alexandre Hainard, Philippe Büscher, John Enyaru, Natalia Tiberti, Gedeao Vatunga |
|---|---|
| Přispěvatelé: | Département de science des protéines humaines [Genève], Université de Genève (UNIGE)-Faculté de médecine [Genève], Department of Biomedical Sciences, Institute of Tropical Medicine [Antwerp] (ITM), Neuroépidémiologie Tropicale (NET), Institut Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST), Université de Limoges (UNILIM)-Université de Limoges (UNILIM)-CHU Limoges-Institut d'Epidémiologie Neurologique et de Neurologie Tropicale-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Limoges (UNILIM), Department of Biotechnical and Diagnostics Sciences, Makerere University [Kampala, Ouganda] (MAK)-College of Veterinary Medicine, Animal Resources and Biosecurity, Department of Biochemistry, College of Natural Sciences-Makerere University [Kampala, Ouganda] (MAK), Neuroscience Department, Karolinska Institutet [Stockholm], Department of Parasitology, Institut National de Recherche Biomédicale, Division of Cellular and Molecular Medicine, St George's University of London, Foundation for Innovative New Diagnostics (FIND) |
| Rok vydání: | 2012 |
| Předmět: |
Male
Trypanosoma brucei gambiense MESH: Trypanosoma brucei gambiense Cohort Studies chemistry.chemical_compound Leukocyte Count 0302 clinical medicine Cerebrospinal fluid Meningoencephalitis African trypanosomiasis MESH: Cohort Studies 0303 health sciences Multidisciplinary biology Neopterin MESH: Immunoglobulin M MESH: Neopterin 3. Good health MESH: Reproducibility of Results Cohort Medicine Biomarker (medicine) Female Adult Science 030231 tropical medicine Trypanosoma brucei 03 medical and health sciences parasitic diseases medicine Humans MESH: Meningoencephalitis ddc:576 030304 developmental biology MESH: Humans MESH: Biological Markers Reproducibility of Results MESH: Adult biology.organism_classification medicine.disease MESH: Male MESH: Trypanosomiasis African Trypanosomiasis African chemistry Immunoglobulin M MESH: Leukocyte Count Immunology biology.protein [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie MESH: Female Biomarkers |
| Zdroj: | PLOS ONE, Vol. 7, No 7 (2012) P. e40909 PLoS ONE PLoS ONE, Public Library of Science, 2012, 7 (7), pp.e40909. ⟨10.1371/journal.pone.0040909⟩ PLoS ONE, Vol 7, Iss 7, p e40909 (2012) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0040909⟩ |
| Popis: | International audience; BACKGROUND: Sleeping sickness, or human African trypanosomiasis (HAT), is a protozoan disease that affects rural communities in sub-Saharan Africa. Determination of the disease stage, essential for correct treatment, represents a key issue in the management of patients. In the present study we evaluated the potential of CXCL10, CXCL13, ICAM-1, VCAM-1, MMP-9, B2MG, neopterin and IgM to complement current methods for staging Trypanosoma brucei gambiense patients. METHODS AND FINDINGS: Five hundred and twelve T. b. gambiense HAT patients originated from Angola, Chad and the Democratic Republic of the Congo (D.R.C.). Their classification as stage 2 (S2) was based on the number of white blood cells (WBC) (>5/µL) or presence of parasites in the cerebrospinal fluid (CSF). The CSF concentration of the eight markers was first measured on a training cohort encompassing 100 patients (44 S1 and 56 S2). IgM and neopterin were the best in discriminating between the two stages of disease with 86.4% and 84.1% specificity respectively, at 100% sensitivity. When a validation cohort (412 patients) was tested, neopterin (14.3 nmol/L) correctly classified 88% of S1 and S2 patients, confirming its high staging power. On this second cohort, neopterin also predicted both the presence of parasites, and of neurological signs, with the same ability as IgM and WBC, the current reference for staging. CONCLUSIONS: This study has demonstrated that neopterin is an excellent biomarker for staging T. b. gambiense HAT patients. A rapid diagnostic test for detecting this metabolite in CSF could help in more accurate stage determination. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|