New insights into early sequential PrPsc accumulation in scrapie infected mouse brain evidenced by the use of streptomycin sulfate
| Autor: | Hervé Perron, Edwige Leclere, Aly Moussa, Anna A. Bencsik |
|---|---|
| Přispěvatelé: | Laboratoire d'études et de recherches en pathologie bovine et hygiène des viandes, Agence Française de Sécurité Sanitaire des Aliments (AFSSA), laboratoire de virologie Médicale, Laboratoire de Virologie Médicale, Recherche & Development (BIOMéRIEUX), bioMérieux SA |
| Rok vydání: | 2008 |
| Předmět: |
Histology
PrPSc Proteins animal diseases Streptomycin Sulfate Scrapie Biology MESH: Streptomycin Microbiology MESH: Brain Mice Cerebrospinal fluid MESH: Early Diagnosis MESH: Mice Inbred C57BL [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases medicine Animals MESH: Animals Prion protein Molecular Biology MESH: Mice [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health MESH: PrPSc Proteins Brain MESH: Immunohistochemistry Cell Biology Virology Immunohistochemistry nervous system diseases Mice Inbred C57BL Medical Laboratory Technology Early Diagnosis nervous system Streptomycin MESH: Scrapie Female MESH: Female medicine.drug Infectious agent |
| Zdroj: | Histochemie / Histochemistry Histochimie Histochemie / Histochemistry Histochimie, 2008, 129 (5), pp.643-50. ⟨10.1007/s00418-008-0382-2⟩ |
| ISSN: | 0948-6143 |
| DOI: | 10.1007/s00418-008-0382-2⟩ |
| Popis: | To investigate the amplifying potentialities of streptomycin sulfate in the immunohistochemical (IHC) detection of the abnormal prion protein (PrPsc), we used a sequential brain sampling from C506M3 scrapie strain inoculated C57Bl/6 mice. The weekly removed brains, from 7 to 63 days post intra-cranial inoculation were analysed using PrPsc IHC. The introduction of streptomycin sulfate, a technique developed for accurate cellular and regional mapping of PrPsc deposition in several animal TSEs, revealed a substantial amplifying effect and a clear specific PrPsc detection as early as 28 days post inoculation. The location of the first detected PrPsc deposits suggests a possible involvement of the cerebrospinal fluid in the early dissemination of the infectious agent. The meaning of these newly accessible PrPsc deposits is discussed in relation to a possible nascent form of PrPsc molecules detected in situ for the first time. Altogether, these findings argue that this method can be highly useful to study the early stages after infection with prion agents. |
| Databáze: | OpenAIRE |
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