Integrating flexible electronics for pulsed electric field delivery in a vascularized 3D glioblastoma model
| Autor: | David Moreau, Martin Baca, Gerwin Dijk, Attila Kaszás, Marie C. Lefevre, Rodney P. O'Connor |
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| Přispěvatelé: | École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT), Ecole Natl Super Mines Gardanne, Institut de Neurosciences de la Timone (INT), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), ANR-18-CE19-0029,FIDELGLIO,Dispositifs bioélectroniques flexibles et implantables pour l'amélioration du traitement du Glioblastome par impulsions électriques(2018) |
| Rok vydání: | 2021 |
| Předmět: |
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
Materials science TK7800-8360 [SDV]Life Sciences [q-bio] Brain tumor Tumor cells 02 engineering and technology 03 medical and health sciences Electric field Cell integrity medicine General Materials Science Electrical and Electronic Engineering Materials of engineering and construction. Mechanics of materials 030304 developmental biology 0303 health sciences Spheroid 021001 nanoscience & nanotechnology medicine.disease Flexible electronics 3. Good health Multiphoton fluorescence microscope TA401-492 Electronics 0210 nano-technology Biomedical engineering Glioblastoma |
| Zdroj: | npj Flexible eletronics npj Flexible eletronics, 2021, 5 (1), pp.19. ⟨10.1038/s41528-021-00115-x⟩ npj Flexible Electronics, Vol 5, Iss 1, Pp 1-9 (2021) |
| ISSN: | 2397-4621 |
| DOI: | 10.1038/s41528-021-00115-x |
| Popis: | Glioblastoma is a highly aggressive brain tumor, very invasive and thus difficult to eradicate with standard oncology therapies. Bioelectric treatments based on pulsed electric fields have proven to be a successful method to treat cancerous tissues. However, they rely on stiff electrodes, which cause acute and chronic injuries, especially in soft tissues like the brain. Here we demonstrate the feasibility of delivering pulsed electric fields with flexible electronics using an in ovo vascularized tumor model. We show with fluorescence widefield and multiphoton microscopy that pulsed electric fields induce vasoconstriction of blood vessels and evoke calcium signals in vascularized glioblastoma spheroids stably expressing a genetically encoded fluorescence reporter. Simulations of the electric field delivery are compared with the measured influence of electric field effects on cell membrane integrity in exposed tumor cells. Our results confirm the feasibility of flexible electronics as a means of delivering intense pulsed electric fields to tumors in an intravital 3D vascularized model of human glioblastoma. |
| Databáze: | OpenAIRE |
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